| 產(chǎn)品編號 | bsm-33082M-Bio |
| 英文名稱 | Mouse Anti-Carbonate dehydratase IX/Biotin Conjugated antibody |
| 中文名稱 | 生物素標記的碳酸酐酶9單克隆抗體 |
| 別 名 | CA IX; CA-IX; CA9; Carbonic anhydrase 9; CAH9_HUMAN; CAIX; Carbonate dehydratase IX; Carbonic anhydrase 9; Carbonic anhydrase IX; Carbonic dehydratase; G250; Membrane antigen MN; MN; P54/58N; P54/58N; pMW1; RCC associated protein G250; RCC-associated antigen G250; Renal cell carcinoma associated antigen G250; Renal cell carcinoma-associated antigen G250. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領域 | 腫瘤 細胞生物 免疫學 激酶和磷酸酶 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | 5H10 |
| 交叉反應 | Human, |
| 產(chǎn)品應用 | WB=1:50-200 ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200 ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 46kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human Carbonic Anhydrase IX (full length) |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein G |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq, Jul 2008] Function: Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia. Subunit: Forms oligomers linked by disulfide bonds. Subcellular Location: Nucleus. Nucleus, nucleolus. Cell membrane; Single-pass type I membrane protein. Cell projection, microvillus membrane; Single-pass type I membrane protein. Note=Found on the surface microvilli and in the nucleus, particularly in nucleolus. Tissue Specificity: Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa. Similarity: Belongs to the alpha-carbonic anhydrase family. Database links: Entrez Gene: 768 Human Entrez Gene: 230099 Mouse Omim: 603179 Human SwissProt: Q16790 Human SwissProt: Q8VHB5 Mouse Unigene: 63287 Human Unigene: 283682 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 碳酸酐酶9（carbonic anhydrase IX，CA IX）是新發(fā)現(xiàn)的碳酸酐酶家族異構體之一，是由酸性氨基酸組成的跨膜糖蛋白，在調控細胞增殖、轉化方面有重要作用。 碳酸酐酶9能催化CO2水解為碳酸和水，參與機體的酸堿平衡，調節(jié)細胞內外pH值，易導致腫瘤的生長和轉移，目前也是子宮腫瘤新的標記物。 |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
