| 產(chǎn)品編號 | bs-19842R-BF488 |
| 英文名稱 | Rabbit Anti-RFWD3/BF488 Conjugated antibody |
| 中文名稱 | BF488標(biāo)記的E3泛素連接酶RFWD3抗體 |
| 別 名 | BC027246; E3 ubiquitin protein ligase RFWD3; E3 ubiquitin-protein ligase RFWD3; FLJ10520; MGC27888; RFWD3; RFWD3_HUMAN; Ring finger and WD repeat domain 3; RING finger and WD repeat domain containing protein 3; RING finger and WD repeat domain-containing protein 3; RING finger protein 201; RNF201. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領(lǐng)域 | 細(xì)胞生物 泛素 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | (predicted: Human, Mouse, Dog, Horse, ) |
| 產(chǎn)品應(yīng)用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 85kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human RFWD3 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
Function: E3 ubiquitin-protein ligase that mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint. May act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome. In response to ionizing radiation, interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53. Subcellular Location: Nucleus. Post-translational modifications: Phosphorylated at Ser-46 and Ser-63 upon DNA damage by ATM or ATR. ATM phosphorylation occurs at early times upon DNA damage, while ATR is the major kinase at later times. Phosphorylation by ATM and ATR is required to stabilize p53/TP53. Similarity: Contains 1 RING-type zinc finger. Contains 3 WD repeats. Database links: Entrez Gene: 55159 Human Omim: 614151 Human SwissProt: Q6PCD5 Human Unigene: 567525 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
