產(chǎn)品編號 | bs-13281R-Cy5.5 |
英文名稱 | Rabbit Anti-phospho-RASA1 (Tyr460)/Cy5.5 Conjugated antibody |
中文名稱 | Cy5.5標記的磷酸化Rho GTP酶激活蛋白1/血管畸形骨肥大綜合征相關蛋白抗體 |
別 名 | GAP (phospho Y460); p-GAP (phospho Y460); Ras GAP; CM AVM; CMAVM; DKFZp434N071; GAP; GTPase activating protein; GTPase-activating protein; OTTHUMP00000222390; OTTHUMP00000222391; OTTHUMP00000222392; OTTHUMP00000222393; p120GAP; p120RASGAP; PKWS; Ras GTPase-activating protein 1; RAS p21 protein activator (GTPase activating protein) 1; Ras p21 protein activator; RASA; RASA1; RASA1_HUMAN; RasGAP; Triphosphatase activating protein. |
規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
說 明 書 | 100ul |
產(chǎn)品類型 | 磷酸化抗體 |
研究領域 | 腫瘤 細胞生物 信號轉(zhuǎn)導 G蛋白偶聯(lián)受體 G蛋白信號 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應 | (predicted: Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Sheep, ) |
產(chǎn)品應用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 116kDa |
性 狀 | Lyophilized or Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthesised phosphopeptide derived from human GAP around the phosphorylation site of Tyr460 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
產(chǎn)品介紹 |
background: The mammalian c-H-, c-K- and N-Ras proto-oncogenes encode ubiquitously expressed proteins (1,2). p21Ras can exist in either a physiologically quiescent GDP-binding state or a GTP-binding signal-emitting state (3). Oncogenic p21Ras proteins are trapped in the excited signal-emitting state because the mechanism normally employed to delimit their excitation period, hydrolysis of their bound GTP to GDP, is impaired as a result of specific mutations (3). Interaction of p21Ras with GTPase activating protein (GAP) can increase hydrolysis of p21Ras-bound GTP by as much as 1000-fold (4,5). The product of the neurofibromatosis type 1 gene (NF1) has also been shown to exhibit p21Ras GAP activity (6,7), and proteins that stimulate the GTPase activity of three other low molecular weight GTPases, including Rho, Rab 3A and Rap 1, have also been described (8,9). Function: Inhibitory regulator of the Ras-cyclic AMP pathway. Stimulates the GTPase of normal but not oncogenic Ras p21. Subunit: Interacts with SQSTM1. Interacts with SPSB1; the interaction does not promote degradation. Interacts with CAV2 (tyrosine phosphorylated form). Directly interacts with NCK1. Interacts with PDGFRB (tyrosine phosphorylated). Interacts (via SH2 domain) with the 'Tyr-9' phosphorylated form of PDPK1. Subcellular Location: Cytoplasm. Tissue Specificity: In placental villi, detected only in the trophoblast layer (cytotrophoblast and syncytiotrophoblast). Not detected in stromal, endothelial or Hofbauer cells (at protein level). DISEASE: Note=Mutations in the SH2 domain of RASA seem to be oncogenic and cause basal cell carcinomas. Defects in RASA1 are the cause of capillary malformation-arteriovenous malformation (CMAVM) [MIM:608354]. CMAVM is a disorder characterized by atypical capillary malformations that are multiple, small, round to oval in shape and pinkish red in color. These capillary malformations are associated with either arteriovenous malformation, arteriovenous fistula, or Parkes Weber syndrome. Defects in RASA1 are a cause of Parkes Weber syndrome (PKWS) [MIM:608355]. PKWS is a disorder characterized by a cutaneous flush with underlying multiple micro-arteriovenous fistulas, in association with soft tissue and skeletal hypertrophy of the affected limb. Similarity: Contains 1 C2 domain. Contains 1 PH domain. Contains 1 Ras-GAP domain. Contains 2 SH2 domains. Contains 1 SH3 domain. Database links: Entrez Gene: 5921 Human Entrez Gene: 218397 Mouse Omim: 139150 Human SwissProt: P20936 Human Unigene: 664080 Human Unigene: 12223 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |
| 无码AV在线免费观看 | 亲妺妺乱的性视频播放 | 中文字幕 好色人妻av | 上海熟妇搡BBBB搡BBBB | 熟女人妻的婬乱和放荡 | 岛国大片在线免费观看 | 无码人妻国产一区二区 | 一区二区三区内射美女毛片 | 国产寡妇亲子伦一区二区三区 | 亚洲 激情 图片 小说 伦 | 黄色视频网站免费观看 | 国产寡妇色XXⅩ交肉视频 | 成人性生交大免费视频 | 欧美最猛黑人XXXX黑人猛交 | 国产伦精品一区二区三区免费 | 成年人免费观看a级毛片 | 国产美女裸体无遮挡免费视频 | 91麻豆精品国产人妻一区二区三区 | 污视频网站在线观看 | 国产奶头好大揉着好爽 | FUCK国产精品一区 | 免费看一级高潮毛片 | 黄色成人视频免费观看 | 海角91成人一区二区三区 | 久久久久亚州Av无吗A片 | 国产精品午夜未成人免费观看 | 欧美日韩中文字幕 | 久久国产精品无码 | 北京熟妇槡BBBB槡BBBB一 | 成人精品一区二区三区中文字幕 | 无码人妻一区二区三区线花季传件 | 国产乱国产乱老熟300部视频 | www.成年女人黄色小视频 | 久久久久亚洲AV成人人电影绿帽人妻 | 成人一区二区在线视频播放免费 | 久久久91人妻无码精品蜜 | 蜜桃视频 一区二区三区 | 3D动漫精品啪啪一区二区免费 | 久久这里只有精品99 | 色诱女教师一区二区三区 |