產(chǎn)品編號 | bs-14300R-FITC |
英文名稱 | Rabbit Anti-DGKK/FITC Conjugated antibody |
中文名稱 | FITC標記的甘油二酯激酶κ/DGK-κ抗體 |
別 名 | 142 kDa diacylglycerol kinase; DAG kinase kappa; DGK kappa; DGKK_HUMAN; Diacylglycerol kinase kappa; Diglyceride kinase kappa. |
規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
說 明 書 | 100ul |
研究領域 | 腫瘤 細胞生物 信號轉導 新陳代謝 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應 | Rat, (predicted: Human, Mouse, ) |
產(chǎn)品應用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 142kDa |
性 狀 | Lyophilized or Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human DGKK |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
產(chǎn)品介紹 |
background: The protein encoded by this gene is an enzyme that phosphorylates diacylglycerol, converting it to phosphatidic acid. The encoded protein is a membrane protein and is inhibited by hydrogen peroxide. Variations in this gene have been associated with hypospadias. [provided by RefSeq, Mar 2011] Function: DGKK (Diacylglycerol kinase kappa) phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). Subunit: Does not form homooligomers. Subcellular Location: Cytoplasm. Membrane; Peripheral membrane protein Tissue Specificity: Expressed in testis, and to a lesser extent in placenta. Post-translational modifications: Phosphorylated at Tyr-78 by some member of the SRC family in response to H(2)O(2). Similarity: Belongs to the eukaryotic diacylglycerol kinase family. Contains 1 DAGKc domain. Contains 1 PH domain. Contains 2 phorbol-ester/DAG-type zinc fingers. Database links: Entrez Gene: 139189 Human Entrez Gene: 331374 Mouse SwissProt: Q5KSL6 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |
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