| 產(chǎn)品編號 | bs-1291R-AP |
| 英文名稱 | Rabbit Anti-ING1/AP Conjugated antibody |
| 中文名稱 | 堿性磷酸酶（AP）標記的生長抑制因子基因1抗體 |
| 別 名 | ING1_HUMAN; Inhibitor of growth protein 1; inhibitor of growth gene 1; Growth inhibitor ING 1; Growth inhibitor ING1; Growth inhibitory protein ING 1; Growth inhibitory protein ING1; Homo sapiens growth inhibitor p33ING1 (ING1) mRNA, complete cds; ING 1; Inhibitor of growth 1; Inhibitor of growth family member 1; Inhibitor of growth protein 1; p24ING1c; p33 ING1; p33ING1; p33ING1b; p47ING1a; Tumor suppressor ING 1; Tumor suppressor ING1. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領域 | 腫瘤 細胞生物 信號轉(zhuǎn)導 細胞凋亡 細胞周期蛋白 轉(zhuǎn)錄調(diào)節(jié)因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | (predicted: Human, Mouse, Rat, ) |
| 產(chǎn)品應用 | WB=1:50-200 IHC-P=1:50-200 IHC-F=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 46kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human inhibitor of growth gene 1 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: This gene encodes a tumor suppressor protein that can induce cell growth arrest and apoptosis. The encoded protein is a nuclear protein that physically interacts with the tumor suppressor protein TP53 and is a component of the p53 signaling pathway. Reduced expression and rearrangement of this gene have been detected in various cancers. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq]. Function: Cooperates with p53/TP53 in the negative regulatory pathway of cell growth by modulating p53-dependent transcriptional activation. Implicated as a tumor suppressor gene. Subcellular Location: Nucleus. Tissue Specificity: Isoform 2 was expressed in all normal tissues and cells examined, as well as in all breast cancer and melanoma cell lines examined. Isoform 3 was expressed in testis, liver, and kidney, weakly expressed in colon and brain and not expressed in breast and cultured melanocytes. Isoform 4 was highly expressed in testis and weakly expressed in brain, but not expressed in breast, colon, kidney, melanocytes, breast cancer or melanoma cell lines. DISEASE: Defects in ING1 are a cause of head and neck squamous cell carcinomas (HNSCC) [MIM:275355]; also known as squamous cell carcinoma of the head and neck. Similarity: Belongs to the ING family. Contains 1 PHD-type zinc finger. Database links: Entrez Gene: 3621 Human Entrez Gene: 26356 Mouse GenBank: NP_937862 Human Omim: 601566 Human SwissProt: Q9UK53 Human SwissProt: Q9QXV3 Mouse Unigene: 46700 Human Unigene: 508725 Human Unigene: 25709 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 腫瘤的發(fā)生是一個多基因參與,多步驟發(fā)生的復雜過程，抑癌基因的功能失活與癌基因的激活為其主要原因之一。 ING1基因是近期發(fā)現(xiàn)的抑癌基因，研究發(fā)現(xiàn)，它的過表達可抑制細胞生長、增殖,促進細胞凋亡，它的低表達與腫瘤的發(fā)生、發(fā)展有關。ING1正常時定位于胞核，發(fā)揮其誘導凋亡和調(diào)控細胞周期的作用，當其易位于胞質(zhì)時上述作用逐步減弱或消失，在腫瘤發(fā)生時，多位于胞漿。 |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
