產(chǎn)品編號 | bs-5187R-Bio |
英文名稱 | Rabbit Anti-phospho-AQP2(Ser269)/Biotin Conjugated antibody |
中文名稱 | 生物素標記的磷酸化水通道蛋白2抗體 |
別 名 | ADH water channel; AQP 2; AQP CD; AQP2; AQPCD; Aquaporin 2 collecting duct; Aquaporin CD; Aquaporin2; Aquaporine 2; Collecting duct water channel protein; MGC34501; Water channel protein for renal collecting duct; WCH CD; WCHCD. |
規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
說 明 書 | 100ul |
產(chǎn)品類型 | 磷酸化抗體 |
研究領域 | 腫瘤 細胞生物 免疫學 通道蛋白 糖尿病 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應 | Rat, (predicted: Mouse, Dog, Pig, Cow, ) |
產(chǎn)品應用 | WB=1:50-200 ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 30kDa |
性 狀 | Lyophilized or Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from mouse AQP2 around the phosphorylation site of Ser269 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
產(chǎn)品介紹 |
background: This gene encodes a water channel protein located in the kidney collecting tubule. It belongs to the MIP/aquaporin family, some members of which are clustered together on chromosome 12q13. Mutations in this gene have been linked to autosomal dominant, and recessive forms of nephrogenic diabetes insipidus. Belongs to the MIP/aquaporin (TC 1.A.8) family. Function: Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Subcellular Location: Apical cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane; Multi-pass membrane protein. Note=Shuttles from vesicles to the apical membrane. Tissue Specificity: Expressed in renal collecting tubules. Post-translational modifications: Ser-256 phosphorylation is necessary and sufficient for expression at the apical membrane. Endocytosis is not phosphorylation-dependent. DISEASE: Defects in AQP2 are the cause of diabetes insipidus nephrogenic autosomal (ANDI) [MIM:125800]; also known as diabetes insipidus nephrogenic type 2. ANDI is caused by the inability of the renal collecting ducts to absorb water in response to arginine vasopressin. It is characterized by excessive water drinking (polydypsia), excessive urine excretion (polyuria), persistent hypotonic urine, and hypokalemia. Inheritance can be autosomal dominant or recessive. Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family. Database links: Entrez Gene: 359 Human Entrez Gene: 11827 Mouse Omim: 107777 Human SwissProt: P41181 Human SwissProt: P56402 Mouse Unigene: 130730 Human Unigene: 20206 Mouse Unigene: 90076 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關(guān)于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |
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