| 產品編號 | bs-0258R-AP |
| 英文名稱 | Rabbit Anti-MAG/AP Conjugated antibody |
| 中文名稱 | 堿性磷酸酶（AP）標記的髓鞘相關糖蛋白-a抗體 |
| 別 名 | Myelin associated glycoprotein; L-MAG; brain-specific neuronal polypeptide 1B236; Myelin-associated glycoprotein; Sialic acid-binding Ig-like lectin 4a; Siglec-4a; Brain neuron cytoplasmic protein 3; MAG_HUMAN; GMA; MAG; S MAG; Siglec 4a; SIGLEC4A. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領域 | 細胞生物 神經生物學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | (predicted: Human, Mouse, Rat, Dog, Cow, Horse, ) |
| 產品應用 | WB=1:50-200 IHC-P=1:50-200 IHC-F=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 67kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MAG |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產品介紹 |
background: MAG (myelin associated glycoprotein) is a Adhesion molecule in postnatal neural development that mediates sialic-acid dependent cell-cell interactions between neuronal and myelinating cells. Preferentially binds to alpha2,3-linked sialic acid. Isoform L-MAG is critical for the formation of myelin in the CNS, whereas isoform S-MAG is sufficient to maintain the integrity of myelin in PNS. Binds to RTN4R. single-pass type I membrane protein. Expressed by myelinating glial cells in the central and peripheral nervous system. Detected in oligodendrocyte processes before formation of compact myelin. Restricted to the periaxonal space after myelination. Isoform S-MAG is the predominant isoform in CNS and PNS of the adult. In CNS isoform L-MAG is the major form synthesized early in development, and it persists as a significant proportion of the MAG present in the adult. In the PNS isoform L-MAG is expressed at modest levels during development; it is absent in the adult. Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. Function: Adhesion molecule in postnatal neural development that mediates sialic-acid dependent cell-cell interactions between neuronal and myelinating cells. Preferentially binds to alpha-2,3-linked sialic acid. Subunit: Binds to RTN4R Subcellular Location: Membrane. Similarity: Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. Contains 4 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 Ig-like V-type (immunoglobulin-like) domain. Database links: Entrez Gene: 4099 Human Entrez Gene: 17136 Mouse Omim: 159460 Human SwissProt: P20916 Human SwissProt: P20917 Mouse Unigene: 643440 Human Unigene: 241355 Mouse Unigene: 87331 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 髓鞘相關糖蛋白（MAG-a）又稱：髓磷脂相關的球蛋白a 髓鞘相關糖蛋白(myelin-associated glycoprotein, MAG)是免疫球蛋白超家族成員，它由中樞神經系統(tǒng)的少突膠質細胞和外周神經系統(tǒng)的施萬細胞表達。 MAG定位于直接和軸突相接觸的髓鞘膜的最里層,它通過介導膠質細胞與軸突的相互作用參與髓鞘的形成及其完整性的維持。同時MAG也是髓鞘來源的神經生長抑制因子的主要成分。在神經系統(tǒng)發(fā)育的不同階段。 MAG顯示不同的功能：即發(fā)育期促進軸突生長,成熟期抑制軸突生長。其抑制作用主要由髓鞘來源的抑制分子的共同受體NgR介導，在神經營養(yǎng)因子受體p75NTR以及小GTP酶Rho等信號分子的共同參與下完成。 |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
