| 產(chǎn)品編號 | bs-1913R-BF647 |
| 英文名稱 | Rabbit Anti-MMP8/BF647 Conjugated antibody |
| 中文名稱 | BF647標(biāo)記的基質(zhì)金屬蛋白酶-8/膠原酶2抗體 |
| 別 名 | Matrilysin/matrix metalloproteinases-8; CLG 1; CLG1; Collagenase II; Collagenase 2; Collagenase neutrophil; HNC; Matrix metalloprotease 8; MMP8; MMP 8; MMP-8; Neutrophil collagenase; PMNL CL; PMNL collagenase; PMNLCL; neutrophil collagenase preproprotein; MMP8_HUMAN; Neutrophil collagenase;Matrix metalloproteinase-8; PMNL-CL. |
| 規(guī)格價格 | 50ug/1680元 購買 100ug/2980元 購買 大包裝/詢價 |
| 說 明 書 | 50ug 100ug |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 神經(jīng)生物學(xué) 信號轉(zhuǎn)導(dǎo) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse, (predicted: Human, Rat, ) |
| 產(chǎn)品應(yīng)用 | Flow-Cyt=1ug/Test IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 38kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MMP8 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the enzyme encoded by this gene is stored in secondary granules within neutrophils and is activated by autolytic cleavage. Its function is degradation of type I, II and III collagens. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008] Function: Can degrade fibrillar type I, II, and III collagens. Subcellular Location: Cytoplasmic granule. Secreted, extracellular space, extracellular matrix (Probable). Note=Stored in intracellular granules. Tissue Specificity: Neutrophils. Similarity: Belongs to the peptidase M10A family. Contains 4 hemopexin-like domains. Database links: Entrez Gene: 4317 Human Entrez Gene: 17394 Mouse Omim: 120355 Human SwissProt: P22894 Human SwissProt: O70138 Mouse Unigene: 161839 Human Unigene: 16415 Mouse Unigene: 44474 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產(chǎn)品圖片 |
Blank control:Mouse spleen.
Primary Antibody (green line): Rabbit Anti-MMP8 antibody (bs-1913R-BF647)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
Blank control: Mouse spleen.
Primary Antibody (green line): Rabbit Anti-MMP8 /AF647 Conjugated antibody (bs-1913R-BF647)
Dilution: 0.2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG-AF647 .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
|
