| 產(chǎn)品編號 | bs-1851R-Bio |
| 英文名稱 | Rabbit Anti-MKP1/Biotin Conjugated antibody |
| 中文名稱 | 生物素標(biāo)記的絲裂原活化蛋白激酶磷酸酶-1抗體 |
| 別 名 | DUSP1; Mitogen activated protein kinase phosphatase 1; CL 100; CL100; Dual Specificity Phosphatase 1; Dual specificity protein phosphatase 1; Dual specificity protein phosphatase hVH1; DUSP 1; DUSP-1; EC 3.1.3.16; EC 3.1.3.48; HVH 1; HVH1; MAP kinase phosphatase 1; MKP 1; MKP1; MKP-1; Protein tyrosine phosphatase CL100; PTPN 10; PTPN10; Serine/threonine specific protein phosphatase; VH 1; VH1; 3ch134; Mkp1; Ptpn16. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領(lǐng)域 | 腫瘤 免疫學(xué) 信號轉(zhuǎn)導(dǎo) 細(xì)胞凋亡 轉(zhuǎn)錄調(diào)節(jié)因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse, Rat, (predicted: Human, Chicken, Dog, Cow, Rabbit, ) |
| 產(chǎn)品應(yīng)用 | WB=1:50-200 ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 39kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DUSP1 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has intrinsic phosphatase activity, and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues. Function: Dual specificity phosphatase that dephosphorylates MAP kinase ERK2 on both 'Thr-183' and 'Tyr-185'. Tissue Specificity: Expressed at high levels in the lung, liver placenta and pancreas. Moderate levels seen in the heart and skeletal muscle. Lower levels found in the brain and kidney. Post-translational modifications: Phosphorylation at Ser-359 and Ser-364 by MAPK1/ERK2 and MAPK3/ERK1 reduces its rate of degradation. Similarity: Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily. Contains 1 rhodanese domain. Contains 1 tyrosine-protein phosphatase domain. Database links: Entrez Gene: 1843 Human Entrez Gene: 19252 Mouse Omim: 600714 Human SwissProt: P28562 Human SwissProt: P28563 Mouse Unigene: 171695 Human Unigene: 239041 Mouse Unigene: 98260 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. MKPs是一類絲氨酸/蘇氨酸和酪氨酸雙重底物特異性的磷酸酶 ,對于絲裂素活化蛋白激酶活性的調(diào)節(jié)起著十分重要的作用，可使絲裂素活化蛋白激酶上的蘇氨酸/酪氨酸去磷酸化失活。目前研究發(fā)現(xiàn)MKPs分別有MKP-1、MKP-2、MKP-3及MKP4-6。 MKPs受MAPK信號通路中多種成分的誘導(dǎo) ,決定了它與MAPK之間作用的特異性。 通過去磷酸化作用調(diào)節(jié)MAPK信號途徑的活性 ,確保了細(xì)胞內(nèi)信號的精確傳遞 ,參與了多種主要的細(xì)胞功能的調(diào)節(jié)。 |
