產(chǎn)品編號(hào) | bsm-41513M |
英文名稱 | SARS-CoV-2 (2019-nCoV) Nucleocapsid Mouse mAb |
中文名稱 | SARS冠狀病毒2核衣殼蛋白單克隆抗體 |
別 名 | SARS-CoV-2 Nucleocapsid Protein; SARS-CoV-2 NP; nucleocapsid protein [Severe acute respiratory syndrome coronavirus 2]; novel coronavirus N Protein; novel coronavirus Nucleocapsid Protein; 2019-nCoV Nucleoprotein; 2019-nCoV N; 2019nCoV N; 2019-nCoV N Protein; 2019 ncov N Protein; 2019-nCoV nucleocapsid protein. |
研究領(lǐng)域 | 細(xì)菌及病毒 |
抗體來(lái)源 | Mouse |
克隆類型 | Monoclonal |
交叉反應(yīng) | SARS-CoV-2 |
產(chǎn)品應(yīng)用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | kDa |
檢測(cè)分子量 | |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | Recombinant SARS-CoV-2 Nucleocapsid protein |
亞 型 | IgG1/kappa |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.2μm filtered solution of PBS (pH7.4). |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
Human Gene ID : SARS-CoV-2 N |
產(chǎn)品圖片 |
Sample:
Lane 1: Recombinant SARS-CoV-2 N protein (WT) (His Tag) (bs-41408P)
Lane 2: Recombinant SARS-CoV-2 N protein (Q9H, P67S, P80R, P151L, S183Y) (His Tag) (bs-41451P)
Lane 3: Recombinant SARS-CoV-2 N protein (D3L, P13T, D103Y, D128Y, H145Y, R203K, G204R, T205I, S235F) (His Tag) (bs-41452P)
Lane 4: Recombinant SARS-CoV-2 N protein (Del204, Del215) (His Tag) (bs-41491P)
Lane 5: Recombinant SARS-Cov-2 N protein (R203M, D377Y) (His Tag) (bs-41492P)
Lane 6: Recombinant SARS-Cov-2 (Omicron, B.1.1.529) N protein (P13L, E31del, R32del, S33del, R203K, G204R) (N-His Tag) (bs-41494P)
Primary: Anti-SARS-CoV-2(2019-nCoV)Nucleocapsid(bsm-41513M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 46 kDa
Observed band size: 50 kDa
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