| 產(chǎn)品編號 | bs-1693R-HRP |
| 英文名稱 | Calnexin |
| 中文名稱 | HRP標記的鈣連蛋白抗體 |
| 別 名 | Calnexin-ER membrane marker; CANX; Cell adhesion molecule with homology to l1cam precursor; CNX; D11Ertd153e; 1110069N15Rik; AI988026; FLJ26570; IP90; P90; Similar to calnexin; Major histocompatibility complex class I antigen-binding protein p88; CALX_HUMAN. |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human, Mouse, Rat (predicted: Rabbit, Cow, Chicken, Horse) |
| 產(chǎn)品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 細胞定位 | 細胞漿 細胞膜 |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CANX: 501-592/592 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq] Function: Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Subunit: Interacts with MAPK3/ERK1. Interacts with KCNH2. Associates with ribosomes. Interacts with SGIP1; involved in negative regulation of endocytosis (By similarity). The palmitoylated form interacts with the ribosome-translocon complex component SSR1, promoting efficient folding of glycoproteins. Subcellular Location: Endoplasmic reticulum membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Post-translational modifications: Phosphorylated at Ser-564 by MAPK3/ERK1. phosphorylation by MAPK3/ERK1 increases its association with ribosomes (By similarity). br>Palmitoylation by DHHC6 leads to the preferential localization to the perinuclear rough ER. It mediates the association of calnexin with the ribosome-translocon complex (RTC) which is required for efficient folding of glycosylated proteins. Similarity: Belongs to the calreticulin family. Database links: 內(nèi)質(zhì)網(wǎng)膜蛋白標志物（ER membrane marker） 鈣連蛋白 calnexin 存在于內(nèi)質(zhì)網(wǎng)中，是內(nèi)質(zhì)網(wǎng)膜標志物，屬于Ca2+的凝集素樣分子伴侶蛋白，可結(jié)合蛋白質(zhì)的寡糖鏈 ，防止蛋白質(zhì)彼此聚集和泛素化，避免折疊不完全的蛋白質(zhì)離開內(nèi)質(zhì)網(wǎng)。 |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
