| 產(chǎn)品編號 | bsm-34142M |
| 英文名稱 | Mouse Anti-OCT2 antibody |
| 中文名稱 | 陽離子轉(zhuǎn)運蛋白2單克隆抗體 |
| 別 名 | organic cation transporter OCT2; POU domain, class 2, transcription factor 2; Octamer-binding transcription factor 2; Oct-2; OTF-2; Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2; POU2F2; NF A2; Oct 2; PO2F2_MOUSE; PO2F2_HUMAN. |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | 13C4 |
| 交叉反應(yīng) | Human,Mouse |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 51 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human OCT2 |
| 亞 型 | IgG2b, Kappa |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Proclin300. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Oct2 is a transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3'). Oct2 regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. It also modulates transcription transactivation by NR3C1, AR and PGR. Oct2 is B cell specific, belongs to the POU transcription factor family class 2 subfamily and contains 1 homeobox domain. Function: Transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3'). Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. Modulates transcription transactivation by NR3C1, AR and PGR. Isoform 5 activates the U2 small nuclear RNA (snRNA) promoter. Subunit: Interacts with NR3C1, AR and PGR. Subcellular Location: Cytoplasm. Nuclear Tissue Specificity: Isoform 3 is B-cell specific. Isoform 5 is expressed in B-cells and the immunoglobulin-expressing T-cell line Molt-4, but not in the T-cell line BW 5147. Similarity: Belongs to the POU transcription factor family. Class-2 subfamily. Contains 1 homeobox DNA-binding domain. Contains 1 POU-specific domain. SWISS: P09086 Gene ID: 5452 Database links: Entrez Gene: 5452 Human Entrez Gene: 18987 Mouse Omim: 164176 Human SwissProt: P09086 Human SwissProt: Q00196 Mouse Unigene: 147381 Human Unigene: 76683 Mouse Unigene: 155998 Rat 同源結(jié)構(gòu)域蛋白（Homeodomain Proteins） OCT-2是B淋巴細胞轉(zhuǎn)錄因子，以免疫球蛋白啟動子為目標，于生發(fā)中心形成和B細胞分化成漿細胞中起作用。 |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (2-Oct) Monoclonal Antibody, Unconjugated (bsm-34142M) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (2-Oct) Monoclonal Antibody, Unconjugated (bsm-34142M) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse lymphoid); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (2-Oct) Monoclonal Antibody, Unconjugated (bsm-34142M) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
