| 產(chǎn)品編號 | bsm-0345M |
| 英文名稱 | Human IgM Mouse mAb |
| 中文名稱 | 人IgM單克隆抗體 |
| 別 名 | human Immunoglobulin M. |
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Specific References (1) | bsm-0345M has been referenced in 1 publications.
[IF=8.025] Shasha Jiang. et al. CRM197-conjugated multi antigen dominant epitope for effective human cytomegalovirus vaccine development. INT J BIOL MACROMOL. 2022 Oct;: ELISA ; Mouse.
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| 研究領(lǐng)域 | 免疫學(xué) |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | 2B8 |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:400-800,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | kDa |
| 檢測分子量 | |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Native Human IgM: full length |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to it's large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. Due to it's high valency IgM is more efficient than other isotypes is binding antigens with repeating epitopes (virus particles and red blood cells) and is more efficient than IgG in activiating the complement pathway. The gene for the mu constant region contains four domains separated by short intervening sequences. Subcellular Location: Isoform 1: Secreted Isoform 2: Cell membrane Gene ID: 3507 |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Human Raji cell lysates
Lane 2: Human RAMOS cell lysates
Lane 3: Human Serum cell lysates
Primary: Anti-Human IgM (bsm-0345M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: kDa
Observed band size: 77 kDa
Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Human IgM) Monoclonal Antibody, Unconjugated (bsm-0345M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
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