| 產(chǎn)品編號(hào) | bs-22698R |
| 英文名稱 | BHMT Rabbit pAb |
| 中文名稱 | BHMT抗體 |
| 別 名 | betaine homocysteine methyltransferase; betaine homocysteine S methyltransferase 1; betaine homocysteine S methyltransferase; betaine--homocysteine S-methyltransferase 1; BHMT1; BHMT1_HUMAN; EC 2.1.1.5; Epididymis secretory sperm binding protein Li 61p; HEL S 61p. |
| 研究領(lǐng)域 | 腫瘤 信號(hào)轉(zhuǎn)導(dǎo) 新陳代謝 |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Pig,Sheep,Cow,Dog) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 45 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human BHMT: 310-406/406 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed. [provided by RefSeq, Jul 2008] Function: Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline. Subcellular Location: Cytoplasm. Tissue Specificity: Found exclusively in liver and kidney. Similarity: Contains 1 Hcy-binding domain. SWISS: Q93088 Gene ID: 635 Database links:
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| 產(chǎn)品圖片 |
Sample:
Lane 1: Mouse Liver Lysates
Lane 2: Rat Liver Lysates
Primary: Anti-BHMT (bs-22698R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45kDa
Observed band size: 45kDa
Paraformaldehyde-fixed, paraffin embedded (Human kidney carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BHMT) Polyclonal Antibody, Unconjugated (bs-22698R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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