| 產(chǎn)品編號(hào) | bs-21579R |
| 英文名稱 | SOX6 Rabbit pAb |
| 中文名稱 | 核轉(zhuǎn)錄因子SOX6抗體 |
| 別 名 | HSSOX 6; HSSOX6; SOX-6 SOX 6; SOX6_HUMAN. |
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Specific References (1) | bs-21579R has been referenced in 1 publications.
[IF=10.19] Xingliang Dai. et al. Crosstalk between microglia and neural stem cells influences the relapse of glioblastoma in GBM immunological microenvironment. CLIN IMMUNOL. 2023 Jun;251:109333 IHC ; Human.
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| 研究領(lǐng)域 | 神經(jīng)生物學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 轉(zhuǎn)錄調(diào)節(jié)因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat,Rabbit,Pig,Sheep,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 92 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SOX6: 351-450/828 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Sox genes comprise a family of genes that are related to the mammalian sex determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. Sox genes encode putative transcriptional regulators implicated in the decision of cell fates during development and the control of diverse developmental processes. The highly complex group of Sox genes cluster at least 40 different loci that rapidly diverged in various animal lineages. At present, 30 Sox genes have been identified. Members of this family have been shown to be conserved during evolution and to play key roles during animal development. Some are involved in human diseases, including sex reversal. Function: Transcriptional activator. Binds specifically to the DNA sequence 5'-AACAAT-3'. Plays a key role in several developmental processes, including neurogenesis and skeleton formation. Subunit: Interacts with DAZAP2 Subcellular Location: Nucleus. Tissue Specificity: Expressed in a wide variety of tissues, most abundantly in skeletal muscle. Post-translational modifications: Sumoylation inhibits the transcriptional activity. Similarity: Contains 1 HMG box DNA-binding domain. Gene ID: 55553 Database links: Entrez Gene: 55553 Human
Entrez Gene: 20679 Mouse Omim: 607257 Human SwissProt: P35712 Human SwissProt: P40645 Mouse SwissProt: Q8BSS6 Mouse Unigene: 368226 Human Unigene: 323365 Mouse
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| 產(chǎn)品圖片 |
Sample:
Lane 1: Human U87MG cell lysates
Lane 2: Human U251 cell lysates
Lane 3: Human HeLa cell lysates
Primary: Anti-SOX6 (bs-21579R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 92 kDa
Observed band size: 100 kDa
Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SOX6) Polyclonal Antibody, Unconjugated (bs-21579R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SOX6 ) Polyclonal Antibody, Unconjugated (bs-21579R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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