產(chǎn)品編號	bsm-33377M
英文名稱	HP1 alpha Mouse mAb
中文名稱	異染色質(zhì)蛋白1-α單克隆抗體
別名	Antigen p25; HP1-α; CBX5; CBX5_HUMAN; Chromobox homolog 5(HP1 alpha homolog, Drosophila); Chromobox homolog 5; Chromobox protein homolog 5; Heterochromatin protein 1 alpha; Heterochromatin protein 1; Heterochromatin protein 1 homolog alpha; HP1 alpha; HP1 alpha homolog; HP1; HP1A; HP1Hs alpha. HP-1-α; HP 1α; HP-1α; HP-1 α; HP1α; HP1 α;
	Specific References (1) \| bsm-33377M has been referenced in 1 publications. [IF=7.666] Sarah C. Wistner. et al. Characterization of Hepatoma-Derived Growth Factor-Related Protein 2 Interactions with Heterochromatin. CELLS-BASEL. 2023 Jan;12(2):325 WB ; Human. PubMed:36672260
研究領(lǐng)域	免疫學轉(zhuǎn)錄調(diào)節(jié)因子
抗體來源	Mouse
克隆類型	Monoclonal
克隆號	7000
交叉反應(yīng)	Human,Mouse (predicted: Rat)
產(chǎn)品應(yīng)用	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
理論分子量	22 kDa
檢測分子量
細胞定位	細胞核
性狀	Liquid
濃度	1mg/ml
免疫原	Recombinant human HP1 alpha Protein: full length
亞型	IgG
純化方法	affinity purified by Protein G
緩沖液	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
產(chǎn)品介紹	HP1 alpha is a component of heterochromatin. It recognizes and binds histone H3 tails methylated at Lys-9, leading to epigenetic repression. HP1 alpha may interact with lamin B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. HP1 proteins are relatively small proteins (~25 kDa) with a conserved amino-terminal chromo domain and a structurally related carboxy-terminal motif, the chromo shadow domain. Both domains of HP1 are required for binding to native chromatin in vivo, but they contribute differentially to binding in euchromatin and heterochromatin. Function: Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Subunit: Interacts with SUV420H1 and SUV420H2. Interacts with HP1BP3. Interacts directly with ATRX, CHAF1A, LBR, NIPBL, SP100, STAM2 and TRIM28 via the chromoshadow domain. Can interact directly with CBX3 via the chromoshadow domain. Interacts with histone H3 methylated at 'Lys-9'. Interacts with BAHD1, MIS12 and DSN1. Interacts with POGZ; POGZ and PXVXL motif-containing proteins such as INCENP and TRIM28 compete for interaction with CBX5. Interacts with INCENP and TRIM24. Interacts with JC virus agnoprotein; this interaction induces the dissociation of CBX5 from LBR, resulting in destabilization of the nuclear envelope. Interacts with CHAMP1. Interacts with ASXL1. Subcellular Location: Nucleus. Chromosome. Chromosome, centromere. Note=Component of centromeric and pericentromeric heterochromatin. Associates with chromosomes during mitosis. Associates specifically with chromatin during metaphase and anaphase. Post-translational modifications: Phosphorylation of HP1 and LBR may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle. Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis. Ubiquitinated. Similarity: Contains 2 chromo domains. SWISS: P45973 Gene ID: 23468 Database links: Entrez Gene: 538885 Cow Entrez Gene: 477593 Dog Entrez Gene: 23468 Human Entrez Gene: 12419 Mouse Entrez Gene: 300266 Rat Omim: 604478 Human SwissProt: P45973 Human SwissProt: Q61686 Mouse Unigene: 349283 Human Unigene: 262059 Mouse
產(chǎn)品圖片	Sample: MOLT-4(Human) Cell Lysate at 30 ug Siha(Human) Cell Lysate at 30 ug A549(Human) Cell Lysate at 30 ug Primary: Anti- HP1 alpha (bsm-33377M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti- Mouse IgG at 1/20000 dilution Predicted band size: 22 kD Observed band size: 22 kD Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HP1 alpha) Monoclonal Antibody, Unconjugated (bsm-33377M) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human Prostate Tumor); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HP1 alpha) Monoclonal Antibody, Unconjugated (bsm-33377M) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HP1 alpha) Monoclonal Antibody, Unconjugated (bsm-33377M) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HP1 alpha) Monoclonal Antibody, Unconjugated (bsm-33377M) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

	AbBy Fluor 350 標記
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	AbBy Fluor 555 標記
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	AbBy Fluor 647 標記
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	APC 標記
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	FITC 標記
	Gold 標記
	HRP 標記
	PE 標記
	PE-Cy3 標記
	PE-CY5 標記
	PE-CY5.5 標記
	PE-CY7 標記
	RBITC 標記

	免疫組化常用試劑
	免疫印跡常用試劑
	各種標記二抗

	1、抗體溶解方法
	2、抗體修復(fù)方式
	3、常用試劑的配制
	4、免疫組化操作步驟
	5、免疫組化問題解答
	6、Western Blotting 操作步驟
	7、Western Blotting 問題解答
	8、關(guān)于肽鏈的設(shè)計
	9、多肽的溶解與保存
	10、酶標抗體效價測定程序