| 產(chǎn)品編號 | bs-20753R |
| 英文名稱 | SOCS3 Rabbit pAb |
| 中文名稱 | 細胞因子信號傳導(dǎo)抑制蛋白3抗體 |
| 別 名 | suppressor of cytokine signaling 3; ATOD4; CIS 3; CIS3; Cish3; Cytokine induced SH2 protein 3; E2a Pbx1 target gene in fibroblasts 10; EF 10; MGC71791; SOCS 3; SOCS-3; SSI 3; SSI3; STAT induced STAT inhibitor 3; Suppressor of cytokine signaling 3; SOCS3_H |
| 研究領(lǐng)域 | 細胞生物 免疫學(xué) 神經(jīng)生物學(xué) 信號轉(zhuǎn)導(dǎo) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Rat (predicted: Mouse,Pig,Cow,Chicken,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1μg/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 25 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SOCS3: 1/80/225 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Cytokines signal transduction pathway suppressor of cytokine signaling 3 positive cells in the brain are widely distributed, some of the cytoplasm of neurons, nerve fibers, glial cell line. suppressor of cytokine signaling 3 studies in recent years to prove: vascular endothelial cells also showed immune SOCS 3-positive. Function: SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity. Suppresses fetal liver erythropoiesis. Regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells. Regulates IL-6 signaling in vivo (By similarity). Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize IL6ST (By similarity). Subunit: Interacts with multiple activated proteins of the tyrosine kinase signaling pathway including IGF1 receptor, insulin receptor and JAK2. Binding to JAK2 is mediated through the KIR and SH2 domains to a phosphorylated tyrosine residue within the JAK2 JH1 domain. Binds specific activated tyrosine residues of the leptin, EPO, IL12, GSCF and gp130 receptors. Interaction with CSNK1E stabilizes SOCS3 protein. Component of the probable ECS(SOCS3) E3 ubiquitin-protein ligase complex which contains CUL5, RNF7/RBX2, Elongin BC complex and SOCS3. Interacts with CUL5, RNF7, TCEB1 and TCEB2. Interacts with CUL2. Subcellular Location: Cytoplasm Tissue Specificity: Widely expressed with high expression in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney. Lower levels in thymus. Post-translational modifications: Phosphorylated on tyrosine residues after stimulation by the cytokines, IL-2, EPO or IGF1. DISEASE: Genetic variation in the promoter region of SOCS3 may be associated with susceptibility to atopic dermatitis 4 (ATOD4) [MIM:605805]. Atopic dermatitis [MIM:603165], also known as eczema commonly begins in infancy or early childhook and is characterized by ichy and inflamed skin. Similarity: Contains 1 SH2 domain. Contains 1 SOCS box domain. SWISS: O14543 Gene ID: 9021 Database links: Entrez Gene: 9021 Human Omim: 604176 Human SwissProt: O14543 Human Unigene: 527973 Human Unigene: 127801 Rat 細胞因子信號轉(zhuǎn)導(dǎo)途徑抑制蛋白SOCS3陽性細胞在腦內(nèi)分布廣泛,陽性產(chǎn)物主要神經(jīng)元核內(nèi);部分神經(jīng)元的胞漿、神經(jīng)纖維、膠質(zhì)細胞。 細胞激酶信號-3抑制劑近年研究證明：血管內(nèi)皮細胞也呈SOCS 3免疫陽性. |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SOCS3) Polyclonal Antibody, Unconjugated (bs-20753R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control (blue line): Hep G2 (fixed with 70% ethanol (Overnight at 4℃) and then permeabilized with 90% methanol for 20 min at -20℃).
Primary Antibody (green line): Rabbit Anti-SOCS3 antibody (bs-20753R),Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, Dilution: 1μg /test.
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