產(chǎn)品編號(hào) | bs-18288R |
英文名稱 | LIPC Rabbit pAb |
中文名稱 | 肝脂酶抗體 |
別 名 | HDLCQ12; Hepatic lipase; Hepatic triacylglycerol lipase; HL; HTGL; Lipase member C; lipC; LIPC_HUMAN; LIPH. |
研究領(lǐng)域 | 心血管 細(xì)胞生物 免疫學(xué) 新陳代謝 |
抗體來(lái)源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應(yīng) | Human,Mouse,Rat (predicted: Sheep,Cow,Dog) |
產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 53 kDa |
檢測(cè)分子量 | |
細(xì)胞定位 | 分泌型蛋白 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human LIPC: 301-400/499 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
LIPC encodes hepatic triglyceride lipase, which is expressed in liver. LIPC has the dual functions of triglyceride hydrolase and ligand/bridging factor for receptor-mediated lipoprotein uptake. [provided by RefSeq, Jul 2008] Function: Hepatic lipase has the capacity to catalyze hydrolysis of phospholipids, mono-, di-, and triglycerides, and acyl-CoA thioesters. It is an important enzyme in HDL metabolism. Hepatic lipase binds heparin. Subcellular Location: Secreted. DISEASE: Defects in LIPC are the cause of hepatic lipase deficiency (HL deficiency) [MIM:151670]. Similarity: Belongs to the AB hydrolase superfamily. Lipase family. Contains 1 PLAT domain. SWISS: P11150 Gene ID: 3990 Database links: Entrez Gene: 3990 Human Omim: 151670 Human SwissProt: P11150 Human Unigene: 654472 Human |
產(chǎn)品圖片 |
Sample:
Lane 1: Mouse Liver tissue lysates
Lane 2: Mouse Kidney tissue lysates
Lane 3: Rat Liver tissue lysates
Lane 4: Rat Kidney tissue lysates
Primary: Anti-LIPC (bs-18288R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 53 kD
Tissue/cell: human liver carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-LIPC Polyclonal Antibody, Unconjugated(bs-18288R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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