| 產(chǎn)品編號 | bs-18235R |
| 英文名稱 | LGI2 Rabbit pAb |
| 中文名稱 | 富含亮氨酸膠質(zhì)瘤失活蛋白2抗體 |
| 別 名 | KIAA1916; Leucine rich glioma inactivated protein 2; Leucine rich repeat LGI family member 2; Leucine-rich glioma-inactivated protein 2; Leucine-rich repeat LGI family member 2; LGI1 like protein 2; LGI1-like protein 2; LGI2; LGI2_HUMAN; LGIL2. |
| 研究領(lǐng)域 | 細(xì)胞生物 免疫學(xué) 神經(jīng)生物學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse (predicted: Human,Rat,Pig,Sheep,Cow,Chicken,GuineaPig,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 60 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human LGI2: 201-300/545 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The leucine-rich (LRR) repeat is a 20-30 amino acid motif that forms a hydrophobic Alpha/Beta horseshoe fold, allowing it to accommodate several leucine residues within a tightly packed core. All LRR repeats contain a variable segment and a highly conserved segment, the latter of which accounts for 11 or 12 residues of the entire LRR motif. LGI2 (leucine-rich repeat LGI family, member 2), also known as KIAA1916 or LGIL2, is a 545 amino acid secreted protein that contains four LRR repeats and seven EAR repeats. Expressed in heart, brain and placenta, LGI2 shares high sequence similarity with other LGI family members and is thought to play a role in the pathogenesis of epileptic disorders. Subcellular Location: Secreted. Tissue Specificity: Brain, heart and placenta. Similarity: Contains 7 EAR repeats. Contains 3 LRR (leucine-rich) repeats. Contains 1 LRRCT domain. Contains 1 LRRNT domain. SWISS: Q8N0V4 Gene ID: 55203 Database links: Entrez Gene: 55203 Human Entrez Gene: 246316 Mouse Omim: 608301 Human SwissProt: Q8N0V4 Human SwissProt: Q8K4Z0 Mouse Unigene: 12488 Human Unigene: 44721 Mouse |
| 產(chǎn)品圖片 |
Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti-LGI2 (bs-18235R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 60 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LGI2) Polyclonal Antibody, Unconjugated (bs-18235R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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