| 產(chǎn)品編號 | bs-17513R |
| 英文名稱 | SLAIN2 Rabbit pAb |
| 中文名稱 | SLAIN2蛋白抗體 |
| 別 名 | FLJ21611; KIAA1458; SLAI2_HUMAN; SLAIN motif containing 2; SLAIN motif family member 2; SLAIN motif-containing protein 2; SLAIN2. |
| 研究領域 | 細胞生物 免疫學 神經(jīng)生物學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse (predicted: Human,Rat,Rabbit,Horse) |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 63 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SLAIN2: 351-450/581 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Binds to the plus end of microtubules and regulates microtubule dynamics and microtubule organization. Promotes cytoplasmic microtubule nucleation and elongation. Required for normal structure of the microtubule cytoskeleton during interphase. Subcellular Location: Cytoplasm > cytoskeleton. Colocalizes with microtubules. Detected at the plus end of growing microtubules. Tissue Specificity: Widely expressed with highest levels in adult liver, testis and ovary, and lowest levels in adult pancreas and spleen and in fetal brain. Post-translational modifications: Is highly phosphorylated during mitosis, but not during interphase. The highly phosphorylated form does not localize at microtubule plus ends and does not interact with MAPRE1 or CKAP5. Similarity: Belongs to the SLAIN motif-containing family. SWISS: Q9P270 Gene ID: 57606 Database links: Entrez Gene: 57606 Human Entrez Gene: 75991 Mouse Omim: 610492 Human SwissProt: Q9P270 Human SwissProt: Q8CI08 Mouse Unigene: 479677 Human Unigene: 486263 Mouse |
| 產(chǎn)品圖片 |
Sample:
Liver (Mouse) Lysate at 40 ug
Testis (Mouse) Lysate at 40 ug
Primary: Anti-SLAIN2 (bs-17513R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 63 kD
Observed band size: 63 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SLAIN2) Polyclonal Antibody, Unconjugated (bs-17513R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
