產(chǎn)品編號(hào) | bs-16063R |
英文名稱 | FCP1 Rabbit pAb |
中文名稱 | FCP1蛋白抗體 |
別 名 | CCFDN; CTD(carboxy-terminal domain, RNA polymerase II, polypeptide A) phosphatase, subunit 1; CTD of POLR2A, phosphatase of, subunit 1; CTDP1; CTDP1_HUMAN; EC 3.1.3.16; RNA polymerase II subunit A C terminal domain phosphatase; RNA polymerase II subunit A |
研究領(lǐng)域 | 細(xì)胞生物 神經(jīng)生物學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 激酶和磷酸酶 表觀遺傳學(xué) |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應(yīng) | Mouse,Rat (predicted: Human,Pig,Sheep,Cow,Chicken,Dog,Horse) |
產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 104 kDa |
檢測分子量 | |
細(xì)胞定位 | 細(xì)胞核 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human FCP1: 201-300/961 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
This gene encodes a protein which interacts with the carboxy-terminus of the RAP74 subunit of transcription initiation factor TFIIF, and functions as a phosphatase that processively dephosphorylates the C-terminus of POLR2A (a subunit of RNA polymerase II), making it available for initiation of gene expression. Mutations in this gene are associated with congenital cataracts, facial dysmorphism and neuropathy syndrome (CCFDN). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Feb 2011] Function: Processively dephosphorylates 'Ser-2' and 'Ser-5' of the heptad repeats YSPTSPS in the C-terminal domain of the largest RNA polymerase II subunit. This promotes the activity of RNA polymerase II. Subcellular Location: Nucleus. Tissue Specificity: Ubiquitously expressed. Isoform 3 is expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and placenta. Post-translational modifications: Phosphorylated. In the presence of TFIIF, the phosphorylated form has an increased CTD phosphatase activity. The phosphorylation is required for the physical interaction with GTF2F1. DISEASE: Defects in CTDP1 are a cause of congenital cataracts facial dysmorphism and neuropathy syndrome (CCFDN) [MIM:604168]. CCFDN is an autosomal recessive developmental disorder that occurs in an endogamous group of Vlax Roma (Gypsies). The syndrome is characterized by a complex clinical phenotype with seemingly unrelated features involving multiple organs and systems. Developmental abnormalities include congenital cataracts and microcorneae, hypomyelination of the peripheral nervous system, impaired physical growth, delayed early motor and intellectual development, facial dysmorphism and hypogonadism. Central nervous system involvement, with cerebral and spinal cord atrophy, may be the result of disrupted development with superimposed degenerative changes. Affected individuals are prone to severe rhabdomyolysis after viral infections and to serious complications related to general anesthesia (such as pulmonary edema and epileptic seizures). Similarity: Contains 1 BRCT domain. Contains 1 FCP1 homology domain. SWISS: Q9Y5B0 Gene ID: 9150 Database links: Entrez Gene: 9150 Human Entrez Gene: 67655 Mouse Entrez Gene: 447713 Xenopus laevis Entrez Gene: 442930 Zebrafish Omim: 604927 Human SwissProt: Q9Y5B0 Human SwissProt: Q7TSG2 Mouse Unigene: 465490 Human Unigene: 312893 Mouse Unigene: 137952 Rat Unigene: 62700 Xenopus laevis Unigene: 133831 Zebrafish |
產(chǎn)品圖片 |
Sample:
Heart(Mouse) Lysate at 40 ug
Primary: Anti- FCP1 (bs-16063R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 104 kD
Observed band size: 104 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FCP1) Polyclonal Antibody, Unconjugated (bs-16063R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
|
| 我想看毛片黄色三级片 | 蜜桃av秘 乱码一区二区三区 | 无码视频在线免费观看 | 91人妻五码一区二区三区 | 亚洲精品久久久久久国产99 | 岳胥乱一区二区三区视频 | 精品国产污污免费网站入口 | 欧美性爱XXXX黑人XYX性爽 | aaaa精品视频蜜桃 | 又粗又大又黄的视频 | 久久免费观看视频 | 国产成人在线免费观看 | 嗯嗯嗯啊啊好爽十八禁网站 | 中国丰满熟女A片免费观 | 国产大片无码一区二区二区 | 国产激情无码AV毛片多多 | chinese麻豆tube人妻 | 河北真实伦对白精彩脏话 | 久久午夜夜伦鲁鲁片免费 | 中文字幕丝袜第一页 | 国产一级A片久久久免费看快餐 | 国产人妻无码一区二区三区 | 国产毛多水多女人A片 | 日本精品久久久久中文人妻 | 中文字幕-区二区三区四区视频 | 国产123区在线观看 午夜成人免费视频网站 | 欧美A级成人婬片免费看 | 国产精品免费一区二区三区在线观看 | EEUSS鲁片一区二区三区四虎 | 黄色视频免费的网站 | www.国产日韩 | 红桃国产精产一区二区三区 | av在线国产国人福利 | 亚洲高清无码在线视频 | 2022精品国偷自产免费观看 | 国产毛片中字幕视频看看 | 国产成人无码精品久久一区二区 | 中国农村特黄A片免费观看 无码免费一区二区三区邵氏 | 国产精品91一区二区 | 国产欧美欧美在线香蕉在线 |