| 產品編號 | bs-14433R |
| 英文名稱 | DSU Rabbit pAb |
| 中文名稱 | 視桿細胞相關蛋白MREG抗體 |
| 別 名 | Dilute suppressor; Dilute suppressor protein homolog; DSU; FLJ10116; MREG_HUMAN; Melanoregulin; MGC90296; MREG; OTTHUMP00000206794; OTTHUMP00000206795; OTTHUMP00000206796; WDT 2; WDT2; Whn dependent transcript 2. |
| 研究領域 | 細胞生物 免疫學 神經(jīng)生物學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse,Rat (predicted: Human,Pig,Cow,Dog,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 25 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DSU: 101-200/214 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The photoreceptor rod cell that is responsible for vision under conditions of low light consists of stacked arrays of disk membranes that make up its outer segment portion. Regulated by complex biochemical mechanisms, the rod outer segment is under constant renewal as new disks form at the base. MREG (melanoregulin), also known as DSU (dilute suppressor protein homolog) or WDT2, is thought to play a role in membrane fusion and in regulating the biogenesis of disk membranes of photoreceptor rods. MREG interacts with RDS (also known as Peripherin-2), a photoreceptor specific tetraspanin protein that is required to maintain normal cell structure during the renewal process of membrane fusion. MREG is 214 amino acids in length, is expressed in photoreceptor cells and and is expressed as two isoforms due to alternative splicing. Function: DSU (Dilute suppressor) is also known as Melanoregulin. It has a role in the incorporation of pigments into hair. It is involved in organelle biogenesis and may function in membrane fusion. It may also regulate the biogenesis of disk membranes, specialized organelles of photoreceptor rod cells. Subcellular Location: Apical cell membrane; Peripheral membrane protein. Note: Localizes to the inner segment and basal outer segment of rods in the retina. Tissue Specificity: Expressed in photoreceptor cells (at protein level). Similarity: Belongs to the melanoregulin family. SWISS: Q8N565 Gene ID: 55686 Database links: Entrez Gene: 55686 Human Entrez Gene: 381269 Mouse Omim: 609207 Human SwissProt: Q8N565 Human SwissProt: Q6NVG5 Mouse |
| 產品圖片 |
Sample:
Embryo (Mouse) Lysate at 40 ug
Primary: Anti- DSU (bs-14433R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 25 kD
Observed band size: 25 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DSU) Polyclonal Antibody, Unconjugated (bs-14433R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
