| 產(chǎn)品編號 | bs-14405R |
| 英文名稱 | DOCK9 Rabbit pAb |
| 中文名稱 | DOCK9蛋白抗體 |
| 別 名 | Cdc42 guanine nucleotide exchange factor zizimin 1; Dedicator of cytokinesis protein 9; DKFZp686C11110; DKFZp686D2047; DKFZp686N04132; DOCK 9; FLJ16744; FLJ44528; DOCK9_HUMAN; FLJ45282; FLJ45601; KIAA1058; KIAA1085; ZIZ1; Zizimin1. |
| 研究領域 | 細胞生物 信號轉導 G蛋白信號 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human (predicted: Mouse,Rat,Pig,Sheep,Cow,Chicken,Dog,Horse) |
| 產(chǎn)品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 236 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DOCK9: 1451-1550/2069 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
DOCK9 is a 2,069 amino acid protein that localizes to the intracytoplasmic membrane and contains one PH domain, one DHR-1 domain and one DHR-2 domain. Expressed in a variety of tissues with highest expression in placenta and heart and lower expression in lung, kidney, brain and skeletal muscle, Zizimin-1 functions as a guanine nucleotide-exchange factor (GEF) that specifically activates Cdc42 by exchanging bound GDP for free GTP. Four isoforms of Zizimin-1 exist due to alternative splicing events. The gene encoding Zizimin-1 maps to human chromosome 13, which houses over 400 genes, such as BRCA2 and RB1, and comprises nearly 4% of the human genome. Trisomy 13, also known as Patau syndrome, is deadly and the few who survive past one year suffer from permanent neurologic defects, difficulty eating and vulnerability to serious respiratory infections. Function: DOCK9 is a guanine nucleotide exchange factor (GEF) that activates CDC42 by exchanging bound GDP for free GTP. Its overexpression induces filopodia formation. Subunit: Homodimer (Probable). Interacts preferentially with nucleotide-depleted CDC42. Subcellular Location: Intracytoplasmic membrane Similarity: Belongs to the DOCK family. Contains 1 DHR-1 domain. Contains 1 DHR-2 domain. Contains 1 PH domain. SWISS: Q9BZ29 Gene ID: 23348 Database links: Entrez Gene: 23348 Human Omim: 607325 Human SwissProt: Q9BZ29 Human |
| 產(chǎn)品圖片 |
Sample: U251 Cell Lysate at 40 ug
Primary: Anti-DOCK9 (bs-14405R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 236 kD
Observed band size: 236 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
