| 產(chǎn)品編號 | bs-13830R |
| 英文名稱 | CELF3 Rabbit pAb |
| 中文名稱 | CELF3蛋白抗體 |
| 別 名 | Bruno-like protein 1; BRUNOL1; CAG repeat protein 4; CAGH4; CELF-3; CELF3; CELF3_HUMAN; CUG-BP-and ETR-3-like factor 3; CUGBP Elav-like family member 3; ELAV-type RNA-binding protein 1; ERDA4; ETR-1; Expanded repeat domain protein CAG/CTG 4; RNA-binding p |
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Specific References (2) | bs-13830R has been referenced in 2 publications.
[IF=2.08] Xianmin Meng. et al. MicroRNA-34a and microRNA-146a target CELF3 and suppress the osteogenic differentiation of periodontal ligament stem cells under cyclic mechanical stretch. J Dent Sci. 2021 Dec;: WB ; Human.
[IF=0] Ma P
et al. Mass spectrometric analysis of active ingredients in fertilized egg for lipid metabolism. 29 January 2020, PREPRINT (Version 1) available at Research Square. WB ; chicken.
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| 研究領域 | 心血管 細胞生物 神經(jīng)生物學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat (predicted: Pig,Cow,Chicken) |
| 產(chǎn)品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 52 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CELF3: 401-465/465 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Feb 2010] Function: RNA-binding protein involved in the regulation of pre-mRNA alternative splicing. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. Binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA. Subcellular Location: Nucleus. Cytoplasm. Tissue Specificity: Expressed in brain. Similarity: Belongs to the CELF/BRUNOL family. Contains 3 RRM (RNA recognition motif) domains. SWISS: Q5SZQ8 Gene ID: 11189 Database links: Entrez Gene: 11189 Human Omim: 612678 Human SwissProt: Q5SZQ8 Human Unigene: 26047 Human |
| 產(chǎn)品圖片 |
Sample: Lovo (human)cell Lysate at 40 ug
Primary: Anti- CELF3(bs-13830R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52kD
Observed band size: 57kD
Sample:
HepG2 (human)cell Lysate at 40 ug
A549 (human)cell Lysate at 40 ug
Primary: Anti- CELF3(bs-13830R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52kD
Observed band size: 57kD
Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Mouse Cerebellum tissue lysates
Lane 3: Rat Cerebrum tissue lysates
Lane 4: Rat Cerebellum tissue lysates
Lane 5: Human SH-SY5Y cell lysates
Primary: Anti-CELF3 (bs-13830R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 42-55 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
