| 產(chǎn)品編號(hào) | bs-10135R |
| 英文名稱 | Caspase-6 subunit p11 Rabbit pAb |
| 中文名稱 | 半胱胺酸蛋白酶蛋白-6抗體 |
| 別 名 | Caspase-6 subunit p11; Caspase-6 p11; Apoptosis Related Cysteine Protease; Apoptotic cysteine protease MCH2; Apoptotic protease MCH 2; Apoptotic protease MCH2; Casp 6; Casp6; Caspase 6 apoptosis related cysteine protease; Caspase 6 precursor; Caspase6; Hu |
| 研究領(lǐng)域 | 細(xì)胞生物 神經(jīng)生物學(xué) 信號(hào)轉(zhuǎn)導(dǎo) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat,Zebrafish (predicted: Pig,Cow,Dog) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 11/33 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Caspase-6 subunit p11: 181-293/293 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Caspase-6 Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death. Subunit is compose of heterodimer of a18 kDa (p18) and a 11 kDa (p11) subunit. Subcellular location at cytoplasmic. Cleavages by CPP32, caspase-8 or -10 generate the two active subunits. It belongs to the peptidase C14 family. Function: Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death. Subunit: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 18 kDa (p18) and a 11 kDa (p11) subunit. Interacts with BIRC6/bruce. Subcellular Location: Cytoplasm. Post-translational modifications: Cleavages by caspase-3, caspase-8 or -10 generate the two active subunits. Similarity: Belongs to the peptidase C14A family. SWISS: P55212 Gene ID: 839 Database links: Entrez Gene: 839 Human Entrez Gene: 12368 Mouse Omim: 601532 Human SwissProt: P55212 Human SwissProt: O08738 Mouse Unigene: 654616 Human Unigene: 281379 Mouse Unigene: 88160 Rat |
| 產(chǎn)品圖片 |
Sample:
Cerebrum (Rat) Lysate at 40 ug
Primary: Anti- Caspase-6 subunit p11 (bs-10135R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 11/33 kD
Observed band size: 33 kD
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Caspase-6 subunit p11) Polyclonal Antibody, Unconjugated (bs-10135R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Caspase-6 subunit p11) Polyclonal Antibody, Unconjugated (bs-10135R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Caspase-6 subunit p11) Polyclonal Antibody, Unconjugated (bs-10135R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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