| 產(chǎn)品編號 | bs-13261R |
| 英文名稱 | GABPA Rabbit pAb |
| 中文名稱 | GA結(jié)合蛋白轉(zhuǎn)錄因子α/GABP-α抗體 |
| 別 名 | E4TF 1A; E4TF1 60; E4TF1A; GA binding protein alpha chain; GA binding protein transcription factor alpha subunit 60kDa; GA binding protein transcription factor alpha subunit; GA-binding protein alpha chain; GABP A; GABP alpha subunit; GABP alpha subunit; |
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Specific References (1) | bs-13261R has been referenced in 1 publications.
[IF=3.738] Rao, Gan. et al. Arsenic Trioxide Triggers Mitochondrial Dysfunction, Oxidative Stress, and Apoptosis via Nrf 2/Caspase 3 Signaling Pathway in Heart of Ducks. BIOL TRACE ELEM RES. Biol Trace Elem Res. 2022 Apr;:1-11 WB ; Duck.
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| 研究領(lǐng)域 | 細胞生物 神經(jīng)生物學 轉(zhuǎn)錄調(diào)節(jié)因子 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Rat (predicted: Mouse,Rabbit,Pig,Sheep,Cow,Chicken,Dog) |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 51 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GABPA: 51-150/454 |
| 亞 型 | |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The transcription factor GA-binding protein (GABP) is composed of two subunits, the Ets-related GABP-alpha and a GABP-alpha-associated subunit, GABP beta. GABP alpha binds to a specific DNA sequence and GABP beta exists as b1 and b2 splice variants that differ in their C-termini. In primary neuronal cultures, GABP beta is expressed in both the cytoplasm and the nucleus, whereas GABP alpha is expressed mainly in the nucleus. GABP is constitutively expressed as either a GABP alpha beta heterodimer or a GABP alpha b heterotetramer, both of which can modify GABP-dependent transcription in vitro and in vivo. The GABP alpha beta tetrameric complex performs many different functions, such as stimulating transcription of the adenovirus E4 gene, differentially activating BRCA1 expression in human breast cell lines, potentiating Tat-mediated activation of long terminal repeat promoter transcription and viral replication in certain cell types, acting as a coordinator of mitochrondrial and nuclear transcription for cytochrome oxidase in neurons and assisting in the regulation of rpL32 gene transcription. Function: Transcription factor capable of interacting with purine rich repeats (GA repeats). Necessary for the expression of the Adenovirus E4 gene. Subunit: Heterotetramer of two alpha and two beta subunits. Subcellular Location: Nucleus. Similarity: Belongs to the ETS family. Contains 1 ETS DNA-binding domain. Contains 1 PNT (pointed) domain. SWISS: Q06546 Gene ID: 2551 Database links: Entrez Gene: 2551 Human Entrez Gene: 14390 Mouse Omim: 600609 Human SwissProt: Q06546 Human SwissProt: Q00422 Mouse Unigene: 473470 Human Unigene: 18974 Mouse Unigene: 145253 Rat |
| 產(chǎn)品圖片 |
Sample:
293T Cell (Human) Lysate at 30 ug
Primary: Anti- GABPA (bs-13261R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51kD
Observed band size: 51kD
Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GABPA) Polyclonal Antibody, Unconjugated (bs-13261R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
