| 產(chǎn)品編號 | bs-12959R |
| 英文名稱 | phospho-ACTH (Ser168) Rabbit pAb |
| 中文名稱 | 磷酸化促腎上腺皮質(zhì)激素ACTH抗體 |
| 別 名 | ACTH(phospho S168); p-ACTH(phospho S168); ACTH; Adrenocorticotropic hormone; Adrenocorticotropin; alpha melanocyte stimulating hormone; alpha-MSH; alphaMSH; beta LPH; beta melanocyte stimulating hormone; beta-endorphin; beta-MSH; CLIP; Corticotropin; Corticotropin lipotropin; Corticotropin-like intermediary peptide; gamma LPH; gamma-MSH; Lipotropin beta; Lipotropin gamma; Lipotropin, included; LPH; Melanocyte-stimulating hormone, included; Melanotropin alpha; Melanotropin beta; Melanotropin gamma; Melanotropin, included; Met-enkephalin; MSH; NPP; POC; POMC; Pomc-1; Pomc1; Pomc2; Pro ACTH endorphin; Pro opiomelanocortin; Pro-opiomelanocortin-alpha; Proopiomelanocortin; Proopiomelanocortin preproprotein; Tetracosactide. |
| 產(chǎn)品類型 | 磷酸化抗體 |
| 研究領(lǐng)域 | 腫瘤 細胞生物 神經(jīng)生物學(xué) 信號轉(zhuǎn)導(dǎo) 新陳代謝 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Rat (predicted: Dog) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 4.5 kDa |
| 檢測分子量 | |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ACTH: DE(p-S)AE |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a polypeptide hormone precursor that undergoes extensive, tissue-specific, post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. There are eight potential cleavage sites within the polypeptide precursor and, depending on tissue type and the available convertases, processing may yield as many as ten biologically active peptides involved in diverse cellular functions. The encoded protein is synthesized mainly in corticotroph cells of the anterior pituitary where four cleavage sites are used; adrenocorticotrophin, essential for normal steroidogenesis and the maintenance of normal adrenal weight, and lipotropin beta are the major end products. In other tissues, including the hypothalamus, placenta, and epithelium, all cleavage sites may be used, giving rise to peptides with roles in pain and energy homeostasis, melanocyte stimulation, and immune modulation. These include several distinct melanotropins, lipotropins, and endorphins that are contained within the adrenocorticotrophin and beta-lipotropin peptides. Mutations in this gene have been associated with early onset obesity, adrenal insufficiency, and red hair pigmentation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008]. Function: ACTH stimulates the adrenal glands to release cortisol. MSH (melanocyte-stimulating hormone) increases thepigmentation of skin by increasing melanin production in melanocytes. Beta-endorphin and Met-enkephalin are endogenous opiates. Subcellular Location: Secreted. Tissue Specificity: ACTH and MSH are produced by the pituitary gland. Post-translational modifications: Specific enzymatic cleavages at paired basic residues yield the different active peptides. O-glycosylated; reducing sugar is probably N-acetylgalactosamine. DISEASE: Defects in POMC may be associated with susceptibility to obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. Defects in POMC are the cause of pro-opiomelanocortinin deficiency (POMCD) [MIM:609734]. Affected individuals present early-onset obesity, adrenal insufficiency and red hair. Similarity: Belongs to the POMC family. SWISS: P01189 Gene ID: 5443 Database links: Entrez Gene: 5443 Human Entrez Gene: 18976 Mouse Omim: 176830 Human SwissProt: P01189 Human SwissProt: P01193 Mouse |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-ACTH (Ser168)) Polyclonal Antibody, Unconjugated (bs-12959R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: rat adrenal tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-phospho-ACTH (Ser168) Polyclonal Antibody, Unconjugated(bs-12959R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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