| 產(chǎn)品編號 | bs-11840R |
| 英文名稱 | FEZ2 Rabbit pAb |
| 中文名稱 | 椎骨蛋白2抗體 |
| 別 名 | Zygin2; Fasciculation and elongation protein zeta 2; Fasciculation and elongation protein zeta-2; FEZ 2; Fez2; FEZ2_HUMAN; Highly similar to zyginII; HUM3CL; MGC117372; Pre T/NK cell associated protein; Zygin 2; Zygin II; Zygin-2. |
| 研究領域 | 細胞生物 神經(jīng)生物學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse,Rat (predicted: Human,Rabbit,Pig,Sheep,Cow,Horse) |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 40 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human FEZ2/Zygin2: 165-240/353 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene is an ortholog of the C. elegans unc-76 gene, which is necessary for normal axonal bundling and elongation within axon bundles. Other orthologs include the rat gene that encodes zygin II, which can bind to synaptotagmin. [provided by RefSeq, Jul 2008] Function: Involved in axonal outgrowth and fasciculation. Subunit: Homodimer; difulfide-linked. May form heterodimers with FEZ1. Interacts with synaptotagmin. Tissue Specificity: Expressed in nonneural tissues, such as heart, lung, spleen, muscle, testis, placenta and melanocytes. Similarity: Belongs to the zygin family. SWISS: Q9UHY8 Gene ID: 9637 Database links: Entrez Gene: 9637 Human Entrez Gene: 225020 Mouse SwissProt: Q76LN0 Human SwissProt: Q9UHY8 Human SwissProt: Q6TYB5 Mouse Unigene: 258563 Human Unigene: 236835 Mouse Unigene: 8714 Rat |
| 產(chǎn)品圖片 |
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Liver (Mouse) Lysate at 40 ug
Primary: Anti- FEZ2 (bs-11840R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40 kD
Observed band size: 46 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (FEZ2) Polyclonal Antibody, Unconjugated (bs-11840R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
