| 產(chǎn)品編號 | bs-11353R |
| 英文名稱 | Munc13 Rabbit pAb |
| 中文名稱 | 神經(jīng)突觸前膜胞內(nèi)蛋白13抗體 |
| 別 名 | Munc 13; KIAA1032; Munc13-1; Protein unc-13 homolog A; UN13A; Unc 13 homolog A; Unc13a; UNC13A; Unc13h1; UN13A_HUMAN. |
| 研究領(lǐng)域 | 腫瘤 細胞生物 神經(jīng)生物學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse,Rat (predicted: Human,Pig,Sheep,Cow,Chicken,Dog) |
| 產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 193 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Munc13 : 1511-1610/1703 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Munc 13s are homologues of the C. elegans unc-13 gene product. Three brain specific isoforms are expressed in rat: Munc 13-1, -13-2, and -13-3. Munc 13-1 is a peripheral membrane protein that is enriched in synaptosomes and localized to plasma membranes but absent from synaptic vesicles. It has multiple regulatory domains (C1 and C2 domains) that may mediate diacylglycerol, phospholipid, and calcium binding. The C terminus of Munc 13-1 interacts directly with a putative coiled coil domain in the N-terminal part of syntaxin. It has been shown that Munc 13 is involved in priming synaptic vesicles. These activated vesicles can be released immediately on Ca2+ influx. Function: Plays a role in vesicle maturation during exocytosis as a target of the diacylglycerol second messenger pathway. Involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and participates in the activity-dependent refilling of readily releasable vesicle pool (RRP). Essential for synaptic vesicle maturation in most excitatory/glutamatergic but not inhibitory/GABA-mediated synapses (By similarity). Also involved in secretory granule priming in insulin secretion (By similarity). Interacts with FBXO45 (via SRY domain); leading to the degradation of UNC13A by the proteasome (By similarity). Subunit: nteracts with the N-termini of STX1A and/or STX1B1 and DOC2A. Interacts with BSN. Interacts with RIMS1 which recruits UNC13A to the active zone. Forms homodimers via its first C2 domain. Also interacts via this domain with the zinc finger domain of RIMS2. Part of a complex consisting of ERC2, RIMS1 and UNC13A. Also part of a complex consisting of UNC13A, RIMS2 and RAB3A (By similarity). Subcellular Location: Cytoplasm. Cell membrane; Peripheral membrane protein. Cell junction; synapse, presynaptic cell membrane. Note: Localized to the active zone of presynaptic density. Translocated to the plasma membrane as response to phorbol ester binding Tissue Specificity: Expressed in pancreatic islet cells. Similarity: Belongs to the unc-13 family. Contains 3 C2 domains. Contains 1 MHD1 (MUNC13 homology domain 1) domain. Contains 1 MHD2 (MUNC13 homology domain 2) domain. Contains 1 phorbol-ester/DAG-type zinc finger. SWISS: Q2TAK8 Gene ID: 84939 Database links: UniProtKB/Swiss-Prot: Q9UPW8.4 |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (Munc13) Polyclonal Antibody, Unconjugated (bs-11353R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (Munc13) Polyclonal Antibody, Unconjugated (bs-11353R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
