| 產(chǎn)品編號 | bs-4239R |
| 英文名稱 | BNIP3 Rabbit pAb |
| 中文名稱 | 促凋亡調(diào)節(jié)蛋白BNIP3抗體 |
| 別 名 | BCL2 Adenovirus E1B 19kDa Interacting Protein 3; BCL2/adenovirus E1B 19 kDa protein interacting protein 3; BCL2/adenovirus E1B 19 kDa protein-interacting protein 3; BNIP 3; BNIP3_HUMAN; NIP 3; NIP3. |
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Specific References (6) | bs-4239R has been referenced in 6 publications.
[IF=5.396] Kaidi Ma. et al. Lactobacillus rhamnosus GG ameliorates deoxynivalenol-induced kidney oxidative damage and mitochondrial injury in weaned piglets. Food Funct. 2022 Mar;: WB ; Piglets.
[IF=5.168] Du et al. MicroRNA-145 induces apoptosis of glioma cells by targeting BNIP3 and Notch signaling. (2017) Oncotarget. 8:61510-61527 IF ; Human.
[IF=4.932] Qifeng Deng. et al. Cordycepin enhances anti-tumor immunity in colon cancer by inhibiting phagocytosis immune checkpoint CD47 expression. Int Immunopharmacol. 2022 Jun;107:108695 WB,IHC ; Mouse.
[IF=4.109] He Bai. et al. Inhibition of the BNIP3/NIX-dependent mitophagy aggravates copper-induced mitochondrial dysfunction in duck renal tubular epithelial cells. ENVIRON TOXICOL. 2022 Nov;: WB ; Duck.
[IF=2.1] Ruiqiao Guan. et al. Electroacupuncture at GV20?GB7 regulates mitophagy to protect against neurological deficits following intracerebral hemorrhage via inhibition of apoptosis. Mol Med Rep. 2021 Jul;24(1):1-15 WB ; Rat.
[IF=1.49] Qian Zhang. et al. Expression of HIF1α, BNIP3, and beclin-1 in the brain of newborn and adult yaks (Bos grunniens). FOLIA HISTOCHEM CYTO. 2023 Mar;61(1):26-33 IHC ; Bovine.
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| 研究領(lǐng)域 | 腫瘤 心血管 免疫學(xué) 細胞凋亡 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat,Pig,Cow,Chicken,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | Flow-Cyt=2ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 22 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human BNIP3: 101-194/194 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Apoptosis-inducing protein that, which can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2. Function: Apoptosis-inducing protein that can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates to mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. Plays an important role in the calprotectin (S100A8/A9)-induced cell death pathway. Subunit: Homodimer. Binds to BCL2. Interacts with BNIP3L and ACAA2. Also can interact with adenovirus E1B 19 kDa protein or Epstein-Barr virus BHRF1. Interacts (via BH3 domain) with SPATA18 (via coiled-coil domains). Subcellular Location: Mitochondrion. Mitochondrion membrane. Coexpression with the EIB 19-kDa protein results in a shift in NIP3 localization pattern to the nuclear envelope. Colocalizes with ACAA2 in the mitochondria. Similarity: Belongs to the NIP3 family. SWISS: Q12983 Gene ID: 664 Database links: Entrez Gene: 664 Human Entrez Gene: 12176 Mouse Omim: 603293 Human SwissProt: Q12983 Human SwissProt: O55003 Mouse Unigene: 144873 Human Unigene: 378890 Mouse Unigene: 2060 Rat BNIP3蛋白在缺氧條件下可誘導(dǎo)細胞發(fā)生細胞凋亡。BNIP3促細胞凋亡線粒體途徑根據(jù)有無caspase的參與而具有不同的作用機制，也是研究的熱點。BNIP3還可誘導(dǎo)細胞自噬。BNIP3全稱為Bcl2/腺病毒E1B19kD相互作用蛋白3。(Bcl2/adenovirusE1B19kDainteractingprotein3，BNIP3)，是新近發(fā)現(xiàn)的BH3only亞家族成員之一，也是低氧敏感的凋亡基因。在化學(xué)性低氧誘導(dǎo)細胞凋亡的過程中，ATP是BNIP3導(dǎo)致細胞凋亡的關(guān)鍵性因素之一。單獨ATP下降不誘導(dǎo)細胞凋亡，但ATP下降到一定程度能促進BNIP3向線粒體轉(zhuǎn)位，從而誘導(dǎo)了BNIP3相關(guān)的凋亡。這可能是低氧誘導(dǎo)BNIP3表達導(dǎo)致細胞凋亡的重要機制之一。 |
| 產(chǎn)品圖片 |
Blank control:A431.
Primary Antibody (green line): Rabbit Anti-BNIP3 antibody (bs-4239R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
