| 產(chǎn)品編號 | bs-5543R |
| 英文名稱 | Phospho-PDPK1 (Tyr9) Rabbit pAb |
| 中文名稱 | 磷酸化3磷酸肌醇依賴性蛋白激酶1抗體 |
| 別 名 | PDPK1(phospho Y9); p-PDPK1(phospho Y9); PDPK1(phospho Tyr9); 3 phosphoinositide dependent protein kinase 1; hPDK 1; hPDK1; MGC20087 MGC35290; PDK 1; PDPK 1; PDPK1; PkB kinase; PkB kinase like gene 1; PkB like 1; PkB like; PRO0461; PDPK1_HUMAN. |
| 產(chǎn)品類型 | 磷酸化抗體 |
| 研究領域 | 免疫學 神經(jīng)生物學 信號轉(zhuǎn)導 細胞凋亡 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse (predicted: Rat) |
| 產(chǎn)品應用 | WB=1:500-2000,ICC/IF=1:25
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 61 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human PDK1 around the phosphorylation site of Tyr9: QL(p-Y)DA |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
PDK1 (3 Phosphoinositide Dependent Protein Kinase 1) phosphorylates AGC kinases. PDK1 activates conventional PKC and PKC zeta through phosphorylation of critical threonine residues in the activation loop. PDK1 also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of phosphatidylinositol-3,4,5-trisphosphate. Active Akt inactivates Glycogen Synthase Kinase 3 (GSK3), eventually leading to the dephosphorylation and activation of glycogen synthase and the stimulation of glycogen synthesis. Because of the role that PDK plays in insulin-induced glycogen synthesis and PKC activation it is a potentially important target for metabolic drug research. There are three named isoforms. Function: Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive. Subunit: Interacts with NPRL2. Subcellular Location: Cytoplasm. Membrane; Peripheral membrane protein. Note=Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane. Tissue Specificity: Appears to be expressed ubiquitously. Post-translational modifications: Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation. Similarity: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily. Contains 1 PH domain. Contains 1 protein kinase domain. SWISS: O15530 Gene ID: 5170 Database links: Entrez Gene: 5170 Human Entrez Gene: 18607 Mouse Omim: 605213 Human SwissProt: O15530 Human SwissProt: Q9Z2A0 Mouse Unigene: 459691 Human Unigene: 10504 Mouse Unigene: 10905 Rat PDK1是蛋白激酶B(PKB)的上游激酶,通過與3,4,5三磷酸磷脂酰肌醇作用激活相鄰的PKB分子.PDK1能夠激活包含PKB在內(nèi)的一系列的AGC激酶家族成員.從而調(diào)節(jié)細胞代謝,生長,擴散,生存,抗凋亡等諸多生理過程. |
| 產(chǎn)品圖片 |
Sample:
Liver (Mouse) Lysate at 40 ug
Heart (Mouse) Lysate at 40 ug
Primary: Anti-Phospho-PDPK1(Tyr9) (bs-5543R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 61 kD
Observed band size: 51 kD
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Phospho-PDPK1 (Tyr9)) polyclonal Antibody, Unconjugated (bs-5543R) 1:25, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
