| 產(chǎn)品編號 | bs-5365R |
| 英文名稱 | phospho-HSP70 (Tyr525) Rabbit pAb |
| 中文名稱 | 磷酸化熱休克蛋白70抗體 |
| 別 名 | HSP70(phospho Tyr525); HSP70(phospho Y525); p-HSP70(Tyr525); p-HSP70(Y525); HSP70; HSP-70; HSP 70; Heat shock 70 kDa protein 1; heat shock 70kDa protein 1A; Heat shock 70kDa protein 1B; Heat shock induced protein; heat shock protein 70; HSP70 1; HSP70 2; |
| 產(chǎn)品類型 | 磷酸化抗體 |
| 研究領(lǐng)域 | 腫瘤 免疫學(xué) 信號轉(zhuǎn)導(dǎo) 轉(zhuǎn)錄調(diào)節(jié)因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human (predicted: Mouse,Rabbit,Pig,Sheep,Cow,Chicken,Dog,GuineaPig) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1μg/Test,ICC/IF=1:100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 70 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human HSP70 around the phosphorylation site of Tyr525: EK(p-Y)KA |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq, Jul 2008]. Function: In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell. Subunit: Component of the CatSper complex. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Interacts with TSC2. Interacts with IRAK1BP1. Interacts with TERT; the interaction occurs in the absence of the RNA component, TERC, and dissociates once the TERT complex has formed. Interacts with DNAJC7. Interacts with CHCHD3. Subcellular Location: Cytoplasm. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Tissue Specificity: HSPA1B is testis-specific. Similarity: Belongs to the heat shock protein 70 family. SWISS: P0DMV9 Gene ID: 3304 Database links: Entrez Gene: 3303 Human Entrez Gene: 3304 Human Entrez Gene: 15511 Mouse Entrez Gene: 193740 Mouse Omim: 140550 Human Omim: 603012 Human SwissProt: P0DMV8 Human SwissProt: P0DMV9 Human SwissProt: P17879 Mouse SwissProt: Q61696 Mouse Unigene: 274402 Human Unigene: 719966 Human Unigene: 728810 Human Unigene: 1950 Rat Unigene: 228225 Rat
HSP-70是細(xì)胞受應(yīng)激原刺激后誘導(dǎo)產(chǎn)生的一組應(yīng)激蛋白，與腫瘤發(fā)生、增殖及分化有關(guān)。環(huán)境和病生理性應(yīng)激原均可導(dǎo)致肌體合成一組應(yīng)急蛋白既熱休克蛋白。許多熱休克蛋白，包括HSP70家族成員，均參與蛋白的變性-復(fù)性、折疊-解折疊、運(yùn)輸-易位、活化-非活化和分泌等過程。 HSP70與類固醇受體、肌動蛋白、P53等蛋白密切相關(guān)。HSP70還參與熱應(yīng)激原、細(xì)胞毒藥物和其他損傷所引起的應(yīng)急反應(yīng)，對機(jī)體起一定的保護(hù)作用。 |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Human skin cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-HSP70 (Tyr525)) Polyclonal Antibody, Unconjugated (bs-5365R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human liver cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-HSP70 (Tyr525)) Polyclonal Antibody, Unconjugated (bs-5365R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (phospho-HSP70 (Tyr525) ) polyclonal Antibody, Unconjugated (bs-5365R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Blank control (blue line): Jurkat (fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice).
Primary Antibody (green line): Rabbit Anti-phospho-HSP70 (Tyr525) antibody (bs-5365R),Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test.
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