| 產(chǎn)品編號(hào) | bs-3482R |
| 英文名稱 | Phospho-cdc25C (Thr48) Rabbit pAb |
| 中文名稱 | 磷酸化細(xì)胞分裂周期蛋白25C抗體 |
| 別 名 | cdc25C(phospho-Thr48); cdc25C(phospho Thr48); cdc25C(phospho T48); CDC 25; Cdc 25C; CDC25; CDC25C; Cell division cycle 25 homolog C; Cell Division Cycle 25C; Cell Division Cycle 25C; Cell division cycle 25C protein; Dual specificity phosphatase Cdc25C; M |
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Specific References (3) | bs-3482R has been referenced in 3 publications.
[IF=5.008] Yin, H., et al. "The molecular mechanism of G2M cell cycle arrest induced by AFB1 in the jejunum." Oncotarget (2016). IHC-P ; Chicken.
[IF=5.008] Guo, Hongrui, et al. "Dietary NiCl2 causes cell cycle arrest in the broiler's kidney." Oncotarget. (2015) 6.34:35964-77. IHC-P ; Chicken.
[IF=2.4] Fang, Jing, et al. "The Molecular Mechanisms of Protective Role of Se on the G2/M Phase Arrest of Jejunum Caused by AFB1." Biological Trace Element Research (2017): 1-12. IHC-P ; Chicken.
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| 產(chǎn)品類型 | 磷酸化抗體 |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 免疫學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 細(xì)胞周期蛋白 轉(zhuǎn)錄調(diào)節(jié)因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 53 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human cdc25C around the phosphorylation site of Thr48: PR(p-T)PV |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known. [provided by RefSeq, Jul 2008] Function: Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. When phosphorylated, highly effective in activating G2 cells into prophase. Directly dephosphorylates CDK1 and activates its kinase activity. Subunit: Interacts with HIV-1 Vpr, thereby inactivating CDC25C phosphatase activity. Interacts with MAPK14 and 14-3-3 proteins. Subcellular Location: Nucleus. Post-translational modifications: Phosphorylated by CHEK1 and MAPK14 at Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase. Phosphorylated by PLK4. Phosphorylated by PLK1, leading to activate the phosphatase activity. Phosphorylation by PLK3 at Ser-191 promotes nuclear translocation. Ser-198 is a minor phosphorylation site. Was initially reported to be phosphorylated by PLK3 at Ser-216 (PubMed:10557092). However, such phosphorylation by PLK3 was not confirmed by other groups. Phosphorylation at Thr-48, Thr-67, Ser-122, Thr-130, Ser-168 and Ser-214 occurs at G2 and G2-M transition and is probably catalyzed by CDK1. Ser-168 phosphorylation levels are lower than those at the other 5 CDK1 sites. Phosphorylation by CDK1 leads to increased activity. Similarity: Belongs to the MPI phosphatase family. Contains 1 rhodanese domain. SWISS: P30307 Gene ID: 995 Database links: Entrez Gene: 995 Human Omim: 157680 Human SwissProt: P30307 Human Unigene: 656 Human CDC25家族的組成哺乳動(dòng)物中CDC25家族一共包括3種同源異構(gòu)體（CDC25A、CDC25B、CDC25C），約有50%的序列同源，是一組在細(xì)胞周期調(diào)控中發(fā)揮巨大作用的蘇/酪氨酸雙功能酶 。不同的CDC25家族蛋白在細(xì)胞周期中的作用時(shí)相亦有差異，CDC25A和CDC25C分別在S期和M期發(fā)揮主要作用。 |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (mouse pancreas tissue); Antigen retrieval by microwave in sodium citratebuffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3%BSA) at RTfor 30min; Antibody incubation with (cdc25C (Thr48)) Polyclonal/MonoclonalAntibody, Unconjugated (bs-3482R) at 1:400 overnight at 4℃,followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by microwave in sodium citratebuffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3%BSA) at RTfor 30min; Antibody incubation with (cdc25C (Thr48)) Polyclonal/MonoclonalAntibody, Unconjugated (bs-3482R) at 1:400 overnight at 4℃,followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by microwave in sodium citratebuffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3%BSA) at RTfor 30min; Antibody incubation with (cdc25C (Thr48)) Polyclonal/MonoclonalAntibody, Unconjugated (bs-3482R) at 1:400 overnight at 4℃,followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Phospho-cdc25C (Thr48)) polyclonal Antibody, Unconjugated (bs-3482R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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