| 產(chǎn)品編號(hào) | bs-3000R |
| 英文名稱 | phospho-14-3-3 protein zeta (Ser58) Rabbit pAb |
| 中文名稱 | 磷酸化14-3-3 α/β/ζ抗體 |
| 別 名 | phospho-14-3-3 protein zeta+delta(Ser58); 14-3-3 zeta(phospho S58); YWHAE(phospho S58); 14-3-3(phospho S58); YWHAZ(phospho S58); 14-3-3 zeta(phospho S58); p-14-3-3 zeta(phospho S58); 14 3 3; 14 3 3 protein beta; 14 3 3 protein beta/alpha; 14 3 3 protein z |
| 產(chǎn)品類型 | 磷酸化抗體 |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 神經(jīng)生物學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 細(xì)胞凋亡 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat,Rabbit,Cow,Chicken,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,Flow-Cyt=1μg /test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 27 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human 14-3-3 protein zeta/delta around the phosphorylation site of Ser58: RS(p-S)WR |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. Because 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. Evidence indicates 14-3-3 is required for Raf 1 kinase activity and phosphorylation amoung many other functions. Function: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Subunit: Interacts with CDK16 and BSPRY. Interacts with WEE1 (C-terminal). Interacts with SAMSN1. Interacts with MLF1 (phosphorylated form); the interaction retains it in the cytoplasm. Interacts with Thr-phosphorylated ITGB2. Interacts with BCL2L11. Homodimer. Heterodimerizes with YWHAE. Homo- and hetero-dimerization is inhibited by phosphorylation on Ser-58. Interacts with FOXO4, NOXA1, SSH1 and ARHGEF2. Interacts with Pseudomonas aeruginosa exoS (unphosphorylated form). Interacts with BAX; the interaction occurs in the cytoplasm. Under stress conditions, MAPK8-mediated phosphorylation releases BAX to mitochondria. Interacts with phosphorylated RAF1; the interaction is inhibited when YWHAZ is phosphorylated on Thr-232. Interacts with TP53; the interaction enhances p53 transcriptional activity. The Ser-58 phosphorylated form inhibits this interaction and p53 transcriptional activity. Interacts with ABL1 (phosphorylated form); the interaction retains ABL1 in the cytoplasm. Interacts with PKA-phosphorylated AANAT; the interaction modulates AANAT enzymatic activity by increasing affinity for arylalkylamines and acetyl-CoA and protecting the enzyme from dephosphorylation and proteasomal degradation. It may also prevent thiol-dependent inactivation. Interacts with AKT1; the interaction phosphorylates YWHAZ and modulates dimerization. Interacts with GAB2 and TLK2. Subcellular Location: Cytoplasm. Melanosome. Note=Located to stage I to stage IV melanosomes. Post-translational modifications: The delta, brain-specific form differs from the zeta form in being phosphorylated (By similarity). Phosphorylation on Ser-184 by MAPK8; promotes dissociation of BAX and translocation of BAX to mitochondria. Phosphorylation on Ser-58 by PKA; disrupts homodimerization and heterodimerization with YHAE and TP53. This phosphorylation appears to be activated by sphingosine. Phosphorylation on Thr-232; inhibits binding of RAF1. Similarity: Belongs to the 14-3-3 family. SWISS: P63104 Gene ID: 7534 Database links: Entrez Gene: 7534 Human Entrez Gene: 22631 Mouse Omim: 601288 Human SwissProt: P63104 Human SwissProt: P63101 Mouse Unigene: 492407 Human Unigene: 3360 Mouse Unigene: 465895 Mouse Unigene: 1292 Rat 信號(hào)傳導(dǎo)（Signaling Intermediates） 14-3-3蛋白是一個(gè)涉及調(diào)節(jié)細(xì)胞凋亡、促細(xì)胞分裂信號(hào)傳導(dǎo)和細(xì)胞周期關(guān)卡的蛋白質(zhì)家族。它被認(rèn)為是通過與絲氨酸殘基磷酸化的蛋白質(zhì)的結(jié)合介導(dǎo)的信號(hào)傳導(dǎo)中的關(guān)鍵調(diào)節(jié)物。通過與Bad(相關(guān)死亡因子)的結(jié)合， 14-3-3 蛋白由于將Bad隔離于胞液而防止了細(xì)胞凋亡。 14-3-3 ζ 蛋白是14-3-3家族成員。它廣泛分布于哺乳動(dòng)物、兩棲類、昆蟲、植物和酵母菌的真核生物高度保守性多功能蛋白質(zhì)。目前已知至少有16個(gè)成員。此抗體識(shí)別分子量為30-31kDa的14-3-3ζ蛋白亞型。 |
| 產(chǎn)品圖片 |
Sample:
Hepg2(Human) Cell Lysate at 30 ug
Primary: Anti-phospho-14-3-3 protein zeta (Ser58) (bs-3000R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 27 kD
Blank control (blue line): A549 (fixed with 70% ethanol (Overnight at 4℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature).
Primary Antibody (green line): Rabbit Anti-phospho-14-3-3 protein zeta + delta (Ser58) antibody (bs-3000R),Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test.
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