| 產(chǎn)品編號(hào) | bs-2504R |
| 英文名稱 | ROS1 Rabbit pAb |
| 中文名稱 | 原癌基因ROS1抗體 |
| 別 名 | ROS; ros protein; c ros; c-ros; c ros1; MCF3; Oncogene ROS; OTTHUMP00000040389; Proto oncogene c ros 1 protein; Proto oncogene tyrosine protein kinase ROS; Proto oncogene tyrosine protein kinase ROS precursor; Ros1; ROS1C; Transmembrane tyrosine specific |
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Specific References (1) | bs-2504R has been referenced in 1 publications.
[IF=8.079] Wang, Ning. et al. Hypoxia drives hematopoiesis with the enhancement of T lineage through eliciting arterial specification of hematopoietic endothelial progenitors from hESC. STEM CELL RES THER. 2022 Dec;13(1):1-20 IF ; Mouse.
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| 研究領(lǐng)域 | 細(xì)胞生物 信號(hào)轉(zhuǎn)導(dǎo) 細(xì)胞凋亡 激酶和磷酸酶 |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,Flow-Cyt=1ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 258 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ROS1: 2231-2347/2347 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This proto-oncogene, highly-expressed in a variety of tumor cell lines, belongs to the sevenless subfamily of tyrosine kinase insulin receptor genes. The protein encoded by this gene is a type I integral membrane protein with tyrosine kinase activity. The protein may function as a growth or differentiation factor receptor. [provided by RefSeq, Jul 2008] Function: Orphan receptor tyrosine kinase (RTK) that plays a role in epithelial cell differentiation and regionalization of the proximal epididymal epithelium. May activate several downstream signaling pathways related to cell differentiation, proliferation, growth and survival including the PI3 kinase-mTOR signaling pathway. Mediates the phosphorylation of PTPN11, an activator of this pathway. May also phosphorylate and activate the transcription factor STAT3 to control anchorage-independent cell growth. Mediates the phosphorylation and the activation of VAV3, a guanine nucleotide exchange factor regulating cell morphology. May activate other downstream signaling proteins including AKT1, MAPK1, MAPK3, IRS1 and PLCG2. Subunit: Interacts with PTPN6 (via SH2 1 domain); the interaction is direct and promotes ROS1 dephosphorylation By similarity. Interacts with PTPN11; may activate the PI3 kinase-mTOR signaling pathway. Interacts with VAV3; constitutive interaction mediating VAV3 phosphorylation. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Tissue Specificity: Expressed in brain. Expression is increased in primary gliomas. Post-translational modifications: Phosphorylated. Probably autophosphorylates. Phosphorylation at Tyr-2274 is required for the interaction with PTPN6 that mediates ROS1 dephosphorylation. Phosphorylation at Tyr-2274 stimulates the kinase activity and the activation of the ERK1 signaling cascade. Phosphorylation at Tyr-2274 and/or Tyr-2334 recruits PTPN11. DISEASE: A chromosomal aberration involving ROS1 is found in a glioblastoma multiforme sample. An intra-chromosomal deletion del6(q21q21) is responsible for the formation of GOPC-ROS1 chimeric protein which is localized to the Golgi and has a constitutive receptor tyrosine kinase activity. A SLC34A2-ROS1 chimeric protein produced in non-small cell lung cancer cells also retains a constitutive kinase activity. A third type of chimeric protein CD74-ROS1 was also identified in those cells. Similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily. Contains 9 fibronectin type-III domains. Contains 1 protein kinase domain. SWISS: P08922 Gene ID: 6098 Database links: Entrez Gene: 6098 Human Omim: 165020 Human SwissProt: P08922 Human Unigene: 1041 Human |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Cerebrum (Rat) Lysate at 40 ug
Lane 2: Cerebellum (Rat) Lysate at 40 ug
Lane 3: Pituitary (Rat) Lysate at 40 ug
Lane 4: Siha (Human) Cell Lysate at 30 ug
Lane 5: U251 (Human) Cell Lysate at 30 ug
Primary: Anti-ROS1 (bs-2504R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 258 kD
Observed band size: >250/110 kD
Blank control:THP-1.
Primary Antibody (green line): Rabbit Anti-ROS1 antibody (bs-2504R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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