| 產品編號 | bs-2033R |
| 英文名稱 | E.coli DH-5 Alpha Rabbit pAb |
| 中文名稱 | DH5α大腸桿菌菌體蛋白抗體 |
| 別 名 | Escherichia coli DH-5 alpha; E.coli DH5 alpha; E.coli DH 5 alpha. |
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Specific References (4) | bs-2033R has been referenced in 4 publications.
[IF=32.086] Zeying Zhang. et al. Rapid Identification and Monitoring of Multiple Bacterial Infections Using Printed Nanoarrays. ADV MATER. 2023 Jan;:2211363 Other ; Escherichia Coli.
[IF=4.916] Li X et al. A novel and facile immunosensor based on a barometer: Application for rapid analysis of Escherichia coli in waters. Talanta, 120859 (2020). Sandwich immunoassays ; E. coli.
[IF=3.998] Bentolhoda Heli. et al. Toward a nanopaper-based and solid phase immunoassay using FRET for the rapid detection of bacteria. Sci Rep-Uk. 2020 Sep;10(1):1-11 E. coli.
[IF=2.596] Zachary D. Call. et al. Paper-based pump-free magnetophoresis. Anal Methods-Uk. 2020 Nov;12(43):5177-5185
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| 研究領域 | 免疫學 細菌及病毒 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | (predicted: Escherichia Coli,DH-5 Alpha) |
| 產品應用 | ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | kDa |
| 檢測分子量 | |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | whole cell protein of Ecoli DH-5 Alpha |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This antibody eacts with Escherichia coli DH5a (E.coli DH5a), Escherichia coli is a gram negative bacillus that belongs to a larger group of Enterobacteriae - bacteria that inhabit the gastrointestinal tract. Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins. 大腸桿菌(E.coli)DH5α菌株是一種廣泛應用于生物制藥的工程菌株,根據(jù)產品質檢要求,需檢測生物制品中DH5α菌體蛋白的殘留量。本產品為用DH5α菌體蛋白為抗原,經免疫家兔獲得的抗血清,在經過親和層析純化而得到抗體IgG，可用于ELISA等實驗檢測。 |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
