| 產(chǎn)品編號(hào) | bs-1533R |
| 英文名稱 | COX4I1 Rabbit pAb |
| 中文名稱 | 細(xì)胞色素c氧化酶IV亞型1抗體 |
| 別 名 | COX41_HUMAN; Cytochrome c oxidase subunit 4 isoform 1, mitochondrial; COX4; Cytochrome c oxidase polypeptide IV; Cytochrome c oxidase subunit IV isoform 1(COX IV-1); COX-IV; COX IV; COX 4; COX 4I1; |
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Specific References (6) | bs-1533R has been referenced in 6 publications.
[IF=6.551] Wei J et al. Endosulfan induces cardiotoxicity through apoptosis via unbalance of pro-survival and mitochondrial-mediated apoptotic pathways. Sci Total Environ
. 2020 Jul 20;727:138790. WB ; human.
[IF=6.025] Xuliang Zhang. et al. PINK1/Parkin-mediated mitophagy mitigates T-2 toxin-induced nephrotoxicity. FOOD CHEM TOXICOL. 2022 Jun;164:113078 WB ; Mouse.
[IF=4.848] Delong Wang. et al. Baoyuan Jiedu Decoction Alleviates Cancer-Induced Myotube Atrophy by Regulating Mitochondrial Dynamics Through p38 MAPK/PGC-1α Signaling Pathway. Front Oncol. 2020; 10: 523577 WB ; Mouse.
[IF=4.451] Jie Gao. et al. Ferulic acid targets ACSL1 to ameliorate lipid metabolic disorders in db/db mice. J Funct Foods. 2022 Apr;91:105009 WB ; Human.
[IF=3.266] Zhang H et al. Cytoplasmic upregulation of Cyto c and AIF serve as biomarkers of mechanical asphyxia death. Am J Transl Res. 2019 Jul 15;11(7):4568-4583. WB ; Rat&Human.
[IF=2.238] Qiao Z et al. Casticin inhibits esophageal cancer cell proliferation and promotes apoptosis by regulating mitochondrial apoptotic and JNK signaling pathways.(2018)Naunyn Schmiedebergs Arch Pharmacol.Nov 17 WB ; Human.
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| 研究領(lǐng)域 | 細(xì)胞生物 免疫學(xué) 線粒體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Pig,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 17 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 細(xì)胞膜 線粒體 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human COX4I1: 101-169/169 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. [provided by RefSeq, Jul 2008] Function: This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. Subcellular Location: Mitochondrion inner membrane. Tissue Specificity: Ubiquitous. Similarity: Belongs to the cytochrome c oxidase IV family. SWISS: P13073 Gene ID: 1327 Database links: Entrez Gene: 1327 Human Entrez Gene: 12857 Mouse SwissProt: P13073 Human SwissProt: P19783 Mouse 細(xì)胞色素c氧化酶4亞型1抗體；（細(xì)胞色素c氧化酶IV亞型1）是一種存在線粒體上的大型跨膜蛋白復(fù)合物。由于細(xì)胞色素氧化酶是呼吸電子傳遞鏈的第四個(gè)中心酶復(fù)合物，因此又被稱為復(fù)合物IV。 |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Human Raji cell lysates
Lane 2: Human HepG2 cell lysates
Lane 3: Human MCF-7 cell lysates
Lane 4: Human A431 cell lysates
Primary: Anti-COX4I1 (bs-1533R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 17 kDa
Observed band size: 15 kDa
Sample: kidney (Mouse) Lysate at 40 ug Primary: Anti-COX4 (Bs-1533R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 17 kD Observed band size: 17 kD
Sample:
heart (Mouse) Lysate at 40 ug
Primary: Anti-COX4 (Bs-1533R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 17 kD
Observed band size: 17 kD
Tissue/cell: mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-COX4/COX IV-1 Polyclonal Antibody, Unconjugated(bs-1533R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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