| 產(chǎn)品編號 | bs-0200R |
| 英文名稱 | PACAP-38 Rabbit pAb |
| 中文名稱 | 腺苷酸環(huán)化酶激活肽-38抗體 |
| 別 名 | HSPC190; Proapoptotic Caspase Adaptor Protein; PACAP; pERp1; Plasma cell-induced ER protein 1; Plasma cell-induced resident endoplasmic reticulum protein; Plasma cell-induced resident ER protein; Proapoptotic caspase adapter protein; pituitary adenylate c |
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Specific References (3) | bs-0200R has been referenced in 3 publications.
[IF=9.58] Ernest Y. Lee. et al. PACAP is a pathogen-inducible resident antimicrobial neuropeptide affording rapid and contextual molecular host defense of the brain. P Natl Acad Sci Usa. 2021 Jan;118(1): IHC ; Mouse.
[IF=8.039] Yifan Zhu. et al. Discovery of Selective P2Y6R Antagonists with High Affinity and In Vivo Efficacy for Inflammatory Disease Therapy. J MED CHEM. 2023;XXXX(XXX):XXX-XXX WB ; Mouse.
[IF=0] PENG Y et al. Effect of electroacupuncture on urodynamics, PACAP-38 and PAC1R of spinal cord in rats with neurogenic bladder ?. World Journal of Acupuncture - Moxibustion,2018 28(1), 50–54. WB ; Rat.
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| 研究領(lǐng)域 | 細胞生物 神經(jīng)生物學 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat (predicted: Pig,Sheep,Cow) |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 5 kDa |
| 檢測分子量 | |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PACAP-38: 31-38/38 (162-139/176) |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Pituitary adenylate cyslase Activating polypeptide-27/38, PACAP-27/38 is a neuropeptide that has substantial sequence homology to vasoactive intestinal peptide (VIP).Both VIPand PACAP-38are equipotent at the VCAP-2 receptor, while PAC AP -38 is equipotent to PACAP-27 and more potent than VIP at the PAC-1receptor. The function of PACAP is Stimulates adenylate cyclase in pituitary cells. It belongs to the glucagon family. Function: Binding to its receptor activates G proteins and stimulates adenylate cyclase in pituitary cells. Subunit: Interacts with ADCYAP1R1 (via N-terminal extracellular domain). Subcellular Location: Secreted. Similarity: Belongs to the glucagon family. SWISS: P18509 Gene ID: 116 Database links: Entrez Gene: 116 Human Entrez Gene: 11516 Mouse Omim: 102980 Human SwissProt: P18509 Human SwissProt: O70176 Mouse Unigene: 531719 Human Unigene: 727476 Human Unigene: 3407 Mouse Unigene: 37400 Rat 腺苷酸環(huán)化酶激活肽是一種可激活垂體腺苷酸環(huán)化酶的多肽，有PACAP-27和 PACAP-38兩種形式，他們共同具有N端27個氨基酸，屬于PACAP/glucagon超級家族。在中樞神經(jīng)系統(tǒng)分布廣泛，其中以下丘腦含量最高。 |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Mouse Pituitary Lysates
Primary: Anti-PACAP-38 (bs-0200R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 5kDa
Observed band size: 19kDa
Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PACPA Polyclonal Antibody, Unconjugated(bs-0200R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PACAP-38 Polyclonal Antibody, Unconjugated(bs-0200R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: mouse small intestine tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PACAP-38 Polyclonal Antibody, Unconjugated(bs-0200R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
