| 產(chǎn)品編號 | bs-0158R |
| 英文名稱 | Neurotrophin 4 Rabbit pAb |
| 中文名稱 | 神經(jīng)生長因子4/5抗體 |
| 別 名 | GLC1O; Neurotrophic factor 4; Neurotrophic factor 5; Neurotrophin 4/5; Neurotrophin 5(neurotrophin 4/5); Neurotrophin 5; Neurotrophin-4; Neurotrophin-5; Neurotrophin4; Neurotrophin4/5; Neurotrophin5; Neutrophic factor 4; Neutrophic factor 5; NT 4; NT-4; N |
| 研究領(lǐng)域 | 細(xì)胞生物 免疫學(xué) 神經(jīng)生物學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Rat (predicted: Mouse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 14/27 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NT-4: 121-210/210 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Neurotrophin 4 (NT4; synonymous with NT5) belongs to the NGF beta family and is a survival factor for peripheral sensory sympathetic neurons. The expression of this gene is ubiquitous and less influenced by environmental signals. NT4 levels are highest in the prostate, with lower levels in thymus, placenta, and skeletal muscle. NT4 is also expressed in embryonic and adult tissues.
Function: Target-derived survival factor for peripheral sensory sympathetic neurons. Subcellular Location: Secreted. Tissue Specificity: Highest levels in prostate, lower levels in thymus, placenta, and skeletal muscle. Expressed in embryonic and adult tissues. DISEASE: Defects in NTF4 may be associated with susceptibility to primary open angle glaucoma type 1O (GLC1O) [MIM:613100]. A form of primary open angle glaucoma (POAG). POAG is characterized by a specific pattern of optic nerve and visual field defects. The angle of the anterior chamber of the eye is open, and usually the intraocular pressure is increased. The disease is asymptomatic until the late stages, by which time significant and irreversible optic nerve damage has already taken place. Similarity: Belongs to the NGF-beta family. SWISS: P34130 Gene ID: 4909 Database links: Entrez Gene: 4909 Human Entrez Gene: 78405 Mouse Omim: 162662 Human SwissProt: P34130 Human SwissProt: Q80VU4 Mouse Unigene: 266902 Human Unigene: 20344 Mouse Unigene: 44225 Rat Neurotrophic factor 4/5起源于同一基因家族， 故可稱為NGF家族。神經(jīng)生長因子4/5具有促進(jìn)早期神經(jīng)元的增殖與分化，維持成熟神經(jīng)元的存活的生物學(xué)功能,能調(diào)控神經(jīng)元再生的微環(huán)境、可促進(jìn)運動神經(jīng)元的存活和生長并能防止它受損及死亡。 |
| 產(chǎn)品圖片 |
Sample: DU145 Cell (Human) Lysate at 40 ug
Primary: Anti-Neurotrophin 4 (bs-0158R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 14/27 kD
Observed band size: 26 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Neurotrophin 4/NT-4/NT-5 Polyclonal Antibody, Unconjugated(bs-0158R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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