| 產(chǎn)品編號 | bs-1067R |
| 英文名稱 | NMDAR2C Rabbit pAb |
| 中文名稱 | 谷氨酸受體2C抗體 |
| 別 名 | NR2C; N-Methyl-d-Asprtate receptor 2C; Glutamate Receptor Ionotropic N Methyl D Aspartate 2C; GRIN2C; N Methly D Aspartate Receptor Channel Subunit Epsilon 3; N Methyl D Aspartate Receptor Subtype 2C; NR2C; NMDE3_HUMAN. |
| 研究領(lǐng)域 | 神經(jīng)生物學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,Flow-Cyt=1ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 132 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NMDAR2C: 601-700/1236 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a subunit of the N-methyl-D-aspartate (NMDA) receptor, which is a subtype of ionotropic glutamate receptor. NMDA receptors are found in the central nervous system, are permeable to cations and have an important role in physiological processes such as learning, memory, and synaptic development. The receptor is a tetramer of different subunits (typically heterodimer of subunit 1 with one or more of subunits 2A-D), forming a channel that is permeable to calcium, potassium, and sodium, and whose properties are determined by subunit composition. Alterations in the subunit composition of the receptor are associated with pathophysiological conditions such as Parkinson's disease, Alzheimer's disease, depression, and schizophrenia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013] Function: NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. Subunit: Interacts with PDZ domains of INADL and DLG4 (By similarity). Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B). Subcellular Location: Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Tissue Specificity: Mainly expressed in brain with predominant expression is in the cerebellum, also present in the hippocampus, amygdala, caudate nucleus, corpus callosum, subthalamic nuclei and thalamus. Detected in the heart, skeletal muscle and pancreas. Similarity: Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2C/GRIN2C subfamily. SWISS: Q14957 Gene ID: 2905 Database links: Entrez Gene: 2905 Human Entrez Gene: 14813 Mouse GenBank: NM_000835 Human GenBank: NM_010350 Mouse Omim: 138254 Human SwissProt: Q14957 Human SwissProt: Q01098 Mouse Unigene: 436980 Human Unigene: 39090 Mouse Unigene: 9709 Rat 谷氨酸受體2C（NR2C 又稱作Glutamate Receptor NMDAR2C;Glutamate receptor ；N-Methyl-d-Asprtate receptor-2C）是脊椎動物中樞神經(jīng)系統(tǒng)興奮型神經(jīng)傳遞的主要介質(zhì)。在突觸可塑性及大腦學(xué)習(xí)及記憶功能方面起關(guān)鍵作用。 |
| 產(chǎn)品圖片 |
Sample:
U251(Human) Cell Lysate at 30 ug
Primary: Anti-NMDAR2C (bs-1067R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 132 kD
Observed band size: 132 kD
Blank control:SH-SY5Y.
Primary Antibody (green line): Rabbit Anti-NMDAR2C antibody (bs-1067R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells wereincubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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