| 產(chǎn)品編號 | bs-0008R |
| 英文名稱 | Nestin Rabbit pAb |
| 中文名稱 | 巢蛋白/神經(jīng)上皮干細胞蛋白抗體 |
| 別 名 | ESTM 46; FLJ 21841; FLJ21841; Intermediate filament protein; Nbla00170; Nes; NEST_RAT; Nestin. |
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Specific References (14) | bs-0008R has been referenced in 14 publications.
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| 研究領(lǐng)域 | 腫瘤 心血管 發(fā)育生物學(xué) 神經(jīng)生物學(xué) 干細胞 細胞類型標(biāo)志物 腫瘤細胞生物標(biāo)志物 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse (predicted: Rat) |
| 產(chǎn)品應(yīng)用 | Flow-Cyt=1μg/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 209 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from rat Nestin: 681-750/1893 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells in the mammalian CNS. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor. Function: Required for brain and eye development (By similarity). Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells. Subunit: Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, this protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3. Subcellular Location: cytoplasm Tissue Specificity: CNS stem cells. Post-translational modifications: Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized. Similarity: Belongs to the intermediate filament family. SWISS: P21263 Gene ID: 25491 Database links: Entrez Gene: 10763 Human Entrez Gene: 18008 Mouse Omim: 600915 Human SwissProt: P48681 Human SwissProt: Q6P5H2 Mouse Unigene: 527971 Human Unigene: 331129 Mouse Unigene: 9701 Rat
神經(jīng)干細胞標(biāo)志物 在動物體早期發(fā)育過程中，中樞神經(jīng)系統(tǒng)發(fā)育尤為重要 。Nestin是一種中等纖維蛋白，它在哺乳動物神經(jīng)前體細胞中表達較高，目前已被廣泛用于神經(jīng)前體細胞的重要標(biāo)志分子。研究Nestin的表達調(diào)控規(guī)律，將有助于人們認(rèn)識神經(jīng)前體細胞的生物特性。此抗體還表達中樞神經(jīng)系統(tǒng)干細胞及神經(jīng)上皮細胞。 |
| 產(chǎn)品圖片 |
Cell: Neuro-2a
Concentration:1:100
Host/Isotype:Rabbit/IgG
Flow cytometric analysis of Rabbit IgG isotype control on Neuro-2a(blue) compared with primary antibody(Cat#: bs-0008R)(green) followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG(H+L) secondary antibody .
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