| 產品編號 | bs-0257R |
| 英文名稱 | MAG Rabbit pAb |
| 中文名稱 | 髓鞘相關糖蛋白a/b抗體 |
| 別 名 | GMA; MAG; MAG_HUMAN; Myelin associated glycoprotein; Myelin-associated glycoprotein; S MAG; Siglec 4a; Siglec-4a; SIGLEC4A. |
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Specific References (3) | bs-0257R has been referenced in 3 publications.
[IF=1.27] Feng, Nianping, et al. "Transplantation of mesenchymal stem cells promotes the functional recovery of the central nervous system following cerebral ischemia by inhibiting myelin?associated inhibitor expression and neural apoptosis." Experimental and Therapeutic Medicine. IHC-P ; Rat.
[IF=1.26] Zhang et al. MicroRNA-210 contributes to peripheral nerve regeneration through promoting the proliferation and migration of Schwann cells. (2017) Exp.Ther.Med. 14:2809-2816 WB ; Rat.
[IF=1.17] Ozgun-Acar, Ozden, et al. "Capparis ovata treatment suppresses inflammatory cytokine expression and ameliorates experimental allergic encephalomyelitis model of multiple sclerosis in C57BL/6 mice." Journal of Neuroimmunology (2016). IHC-P ; Mouse.
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| 研究領域 | 細胞生物 神經生物學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human (predicted: Mouse,Rat) |
| 產品應用 | WB=1:500-2000,Flow-Cyt=1ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 67 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MAG-a/b: 501-582/582 <Cytoplasmic> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
MAG (myelin associated glycoprotein) is a Adhesion molecule in postnatal neural development that mediates sialic-acid dependent cell-cell interactions between neuronal and myelinating cells. Preferentially binds to alpha2,3-linked sialic acid. Isoform L-MAG is critical for the formation of myelin in the CNS, whereas isoform S-MAG is sufficient to maintain the integrity of myelin in PNS. Binds to RTN4R. single-pass type I membrane protein. Expressed by myelinating glial cells in the central and peripheral nervous system. Detected in oligodendrocyte processes before formation of compact myelin. Restricted to the periaxonal space after myelination. Isoform S-MAG is the predominant isoform in CNS and PNS of the adult. In CNS isoform L-MAG is the major form synthesized early in development, and it persists as a significant proportion of the MAG present in the adult. In the PNS isoform L-MAG is expressed at modest levels during development; it is absent in the adult. Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. Function: Adhesion molecule in postnatal neural development that mediates sialic-acid dependent cell-cell interactions between neuronal and myelinating cells. Preferentially binds to alpha-2,3-linked sialic acid. Subunit: Binds to RTN4R. Subcellular Location: Membrane; Single-pass type I membrane protein. Similarity: Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. Contains 4 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 Ig-like V-type (immunoglobulin-like) domain. SWISS: P20916 Gene ID: 4099 Database links: Entrez Gene: 4099 Human Entrez Gene: 17136 Mouse Omim: 159460 Human SwissProt: P20916 Human SwissProt: P20917 Mouse Unigene: 643440 Human Unigene: 241355 Mouse Unigene: 87331 Rat 髓鞘相關糖蛋白a/b（MAG-a/b）又稱：髓磷脂相關的球蛋白 髓鞘相關糖蛋白(myelin-associated glycoprotein, MAG)是免疫球蛋白超家族成員，它由中樞神經系統(tǒng)的少突膠質細胞和外周神經系統(tǒng)的施萬細胞表達。 MAG定位于直接和軸突相接觸的髓鞘膜的最里層，它通過介導膠質細胞與軸突的相互作用參與髓鞘的形成及其完整性的維持。同時MAG也是髓鞘來源的神經生長抑制因子的主要成分。在神經系統(tǒng)發(fā)育的不同階段，MAG顯示不同的功能:即發(fā)育期促進軸突生長，成熟期抑制軸突生長。其抑制作用主要由髓鞘來源的抑制分子的共同受體NgR介導，在神經營養(yǎng)因子受體p75NTR以及小GTP酶Rho等信號分子的共同參與下完成。 |
| 產品圖片 |
Sample: U251 Cell Lysate at 30 ug
Primary: Anti-MAG (bs-0257R) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution;
Predicted band size:67 kD
Observed band size:67 kD
Blank control:SH-SY5Y.
Primary Antibody (green line): Rabbit Anti-MAG antibody (bs-0257R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
