| 產(chǎn)品編號 | bs-1070R |
| 英文名稱 | NPY1R Rabbit pAb |
| 中文名稱 | 神經(jīng)肽Y1受體抗體 |
| 別 名 | neuropeptide Y1 receptor; Npy1r; neuropeptide Y receptor Y1; MGC109393; NPY-1; NPY1R_HUMAN. |
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Specific References (3) | bs-1070R has been referenced in 3 publications.
[IF=5.711] Kang X et al. Neuropeptide Y Acts Directly on Cartilage Homeostasis and Exacerbates Progression of Osteoarthritis through NPY2R. J Bone Miner Res. 2020 Feb 26. IHC,WB ; mouse.
[IF=5.682] Tüfekci, K?ymet Kübra. et al. A Maternal High-Fat Diet Causes Anxiety-Related Behaviors by Altering Neuropeptide Y1 Receptor and Hippocampal Volumes in Rat Offspring: the Potential Effect of N-Acetylcysteine. MOL NEUROBIOL. 2022 Dec;:1-16 IHC ; Rat.
[IF=0] 朱镕鑫, et al. "Ghrelin 信號通路在有氧運(yùn)動對高膽固醇血癥小鼠心肌損傷保護(hù)中的作用." WB ; Mouse.
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| 研究領(lǐng)域 | 神經(jīng)生物學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Rabbit,Pig,Dog) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 44 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NPY1R: 201-300/382 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Neuropeptide Y (NPY) is one of the most abundant neuropeptides in the mammalian nervous system and exhibits a diverse range of important physiologic activities, including effects on psychomotor activity, food intake, regulation of central endocrine secretion, and potent vasoactive effects on the cardiovascular system. There are two major subtypes of NPY (Y1 and Y2). The NPY Y1 receptors have been identified in a variety of tissues, including brain, spleen, small intestine, kidney, testis, placenta, breast, colon, ganglion, heart, and aortic smooth muscle. The Y2 receptor is found mainly in the central nervous system. NPY receptors are a member of the G protein-coupled receptor superfamily. Function: Receptor for neuropeptide Y and peptide YY. The rank order of affinity of this receptor for pancreatic polypeptides is NPY > [Pro-34] PYY, PYY and [Leu-31, Pro-34] NPY > NPY (2-36) > [Ile-31, Gln-34] PP and PYY (3-36) > PP > NPY free acid. Subcellular Location: Cell membrane. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: P25929 Gene ID: 4886 Database links: Entrez Gene: 4886 Human Entrez Gene: 18166 Mouse Omim: 162641 Human SwissProt: P25929 Human SwissProt: Q04573 Mouse Unigene: 519057 Human Unigene: 5112 Mouse Unigene: 11642 Rat |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Mouse NIH/3T3 tissue lysates
Lane 2: Rat Cerebrum tissue lysates
Lane 3: Rat Kidney tissue lysates
Lane 4: Human 293T cell lysates
Primary: Anti-NPY1R (bs-1070R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 60 kDa
Tissue/cell: rat myocardium tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-NPY1R Polyclonal Antibody, Unconjugated(bs-1070R) 1:400, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
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