| 產品編號 | bs-0736R |
| 英文名稱 | CD56 Rabbit pAb |
| 中文名稱 | 神經細胞粘附分子1抗體 |
| 別 名 | CD56 antigen; Cell Adhesion Molecule Neural 1; MSK39; N CAM 120; N CAM 140; N CAM 180; NCAM 1; NCAM 120; NCAM 140; NCAM1; Neural cell adhesion molecule 1 120 kDa isoform; Neural cell adhesion molecule 1 140 kDa isoform; Neural cell adhesion molecule 1; NCAM1_HUMAN. |
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Specific References (3) | bs-0736R has been referenced in 3 publications.
[IF=5.68] Shi, Liang, et al. "Targeting Androgen Receptor (AR)→ IL12A Signal Enhances Efficacy of Sorafenib Plus NK Cells-Immunotherapy to Better Suppress HCC Progression." Molecular Cancer Therapeutics (2016): molcanther-0706. IHC-P ; Human.
[IF=3.886] Huiqian Zeng. et al. Increased uterine NLRP3 inflammasome and leucocyte infiltration in a rat model of preeclampsia. 2021 Aug 10 IHC ; Rat.
[IF=3.26] Liu, Xin-Qi, et al. "Regulation of neuroendocrine cells and neuron factors in the ovary by zinc oxide nanoparticles." Toxicology Letters (2016). IHC-P ; Chicken.
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| 研究領域 | 細胞生物 神經生物學 干細胞 細胞類型標志物 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Rat (predicted: Mouse,Rabbit,Pig,Cow,Chicken,Dog,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 92 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CD56/NCAM1: 701-800/858 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2011] Function: This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. Subcellular Location: Isoform 1, 2,: Cell membrane; Single-pass type I membrane protein. Isoform 3, 4: Cell membrane; Lipid-anchor, GPI-anchor. Isoform 5, 6: Secreted. Similarity: Contains 2 fibronectin type-III domains. Contains 5 Ig-like C2-type (immunoglobulin-like) domains. SWISS: P13591 Gene ID: 4684 Database links: Entrez Gene: 4684 Human Entrez Gene: 17967 Mouse Omim: 116930 Human SwissProt: P13591 Human SwissProt: P13595 Mouse Unigene: 503878 Human Unigene: 711235 Human Unigene: 733031 Human Unigene: 439182 Mouse Unigene: 4974 Mouse Unigene: 11283 Rat 細胞粘附蛋白（Call Adhesion Protein） 神經元標志物 NCAM-1為神經細胞粘附分子，主要分布于神經組織，神經—肌肉接頭，神經—內分泌腺和某些內分泌腺以及大多數(shù)神經外胚層來源的細胞、組織和腫瘤中。 NCAM 1主要用于視網(wǎng)膜母細胞瘤、髓母細胞瘤、星形細胞瘤、神經母細胞瘤等腫瘤方面的研究。CD56（神經細胞黏附分子，NCAM）表達于大部分神經外胚層來源的細胞系、組織和腫瘤如視網(wǎng)膜母細胞瘤、成神經管細胞瘤、星形細胞瘤以及成神經細胞瘤。它也表達于一些中胚層來源的腫瘤如橫紋肌肉瘤。NK細胞以及NK細胞淋巴瘤為陽性。CD56被認為是NK細胞腫瘤的重要標志物。該抗體識別分子量為180，145和125kDa亞型。 |
| 產品圖片 |
Protein: Hela(human)cell lysates at 40ug;
Primary: rabbit Anti-CD56 (bs-0736R) at 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000;
Predicted band size:92 kD
Observed band size:125 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-NCAM1 Polyclonal Antibody, Unconjugated(bs-0736R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
