| 產(chǎn)品編號 | bs-0576R |
| 英文名稱 | Annexin A2 Rabbit pAb |
| 中文名稱 | 膜粘連蛋白 Ⅱ抗體 |
| 別 名 | ANX 2; ANX2; ANX2L4; ANXA2; arylsulfatase B; CAL1H; Calpactin I heavy chain; calpactin I heavy polypeptide(p36); chromobindin 8; LIP2; Lipocortin II; LPC2; LPC2D; P36; P36 protein; PAP-IV; placental anticoagulant protein IV; Protein I; ANXA2_HUMAN; Annexi |
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Specific References (2) | bs-0576R has been referenced in 2 publications.
[IF=2.84] Geng, Xiaofang, et al. "Differential proteome analysis of the cell differentiation regulated by BCC, CRH, CXCR4, GnRH, GPCR, IL1 signaling pathways in Chinese fire-bellied newt limb regeneration." Differentiation (2014). WB ;
[IF=1.832] Gao J et al. Proteomic Analyses of Mammary Glands Provide Insight into the Immunity and Metabolism Pathways Associated with Clinical Mastitis in Meat Sheep.Animals (Basel). 2019 May 31;9(6). pii: E309. WB ; Sheep.
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| 研究領(lǐng)域 | 腫瘤 心血管 細胞生物 信號轉(zhuǎn)導(dǎo) 細胞凋亡 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Sheep,Cow,Chicken,Dog) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 36 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 細胞膜 細胞外基質(zhì) 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Annexin II: 256-339/339 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. This gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]. Function: Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response. Subunit: Heterotetramer containing 2 light chains of S100A10/p11 and 2 heavy chains of ANXA2/p36. Interacts with ATP1B1 and DYSF. Interacts with human cytomegalovirus (HCMV). Interacts with COCH. Subcellular Location: Secreted, extracellular space, extracellular matrix, basement membrane. Melanosome. Note=In the lamina beneath the plasma membrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Translocated from the cytoplasm to the cell surface through a Golgi-independent mechanism. Post-translational modifications: Phosphorylation of Tyr-24 enhances heat stress-induced translocation to the cell surface. ISGylated. Similarity: Belongs to the annexin family. Contains 4 annexin repeats. SWISS: P07355 Gene ID: 302 Database links: Entrez Gene: 302 Human Entrez Gene: 12306 Mouse Omim: 151740 Human SwissProt: P07355 Human SwissProt: P07356 Mouse Unigene: 511605 Human Unigene: 238343 Mouse Unigene: 90546 Rat 信號傳導(dǎo)（Signaling Intermediates） Annexin 2是新近發(fā)現(xiàn)的纖維蛋白溶解酶原(PLG)和組織型纖溶蛋白溶解酶原激活物(t-PA)的共受體,是Ca2+介導(dǎo)具有高親和力的磷脂結(jié)合蛋白.其介導(dǎo)血管內(nèi)外纖維蛋白溶解,對保持機體內(nèi)環(huán)境穩(wěn)定具有重要作用. 依賴性膜磷脂結(jié)合蛋白Annexin II調(diào)控異常及有害因子的作用,可導(dǎo)致血栓性疾病和動脈硬化癥 鈣依賴性膜磷脂結(jié)合蛋白膜粘連蛋白2異常高表達所引起纖溶亢進,是白血病等惡性腫瘤出血合并癥重要機制之一.鈣依賴性磷脂結(jié)合蛋白對其干預(yù)和調(diào)控可能為治療上述病癥提供新思路,為臨床治療開辟新途徑. |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Mouse Kidney tissue lysates
Lane 2: Mouse Esophagus tissue lysates
Lane 3: Mouse Cerebrum tissue lysates
Lane 4: Rat Kidney tissue lysates
Lane 5: Rat Liver tissue lysates
Lane 6: Rat Esophagus tissue lysates
Lane 7: Rat Cerebrum tissue lysates
Lane 8: Human MCF-7 cell lysates
Lane 9: Human K562 cell lysates
Lane 10: Human HUVEC cell lysates
Lane 11: Human HeLa cell lysates
Primary: Anti-Annexin A2 (bs-0576R) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
